Anti-LHX5 Rabbit Polyclonal Antibody for WB and ELISA - Q9H2C1

Core Product Specifications and Parameters

Biological Background: LHX5 Function and Localization

• LIM/homeobox protein Lhx5 (LHX5) is a transcription factor characterized by two N-terminal LIM domains and a central homeodomain.
• It plays an essential role in regulating neuronal differentiation and migration during central nervous system development.
• LHX5 belongs to the LIM-homeodomain family of transcription factors, which are critical for cell fate determination and tissue patterning.
• The protein localizes predominantly to the nucleus, consistent with its function in transcriptional regulation.
• It is expressed in the fetal brain and in specific regions of the adult central nervous system, including the spinal cord, thalamus, and cerebellum, suggesting roles in both development and adult brain function.
• The LIM domains facilitate protein-protein interactions and are zinc-binding, while the homeodomain mediates sequence-specific DNA binding.
• Keywords associated with LHX5 include DNA-binding, transcription regulation, nucleus, metal-binding, and LIM domain, underscoring its role as a transcriptional regulator.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 120–180 of human LHX5, a region near the homeodomain and between the two LIM domains. This may enable recognition of multiple species and potentially different phosphorylation or interaction states.
• For western blot (WB) applications, a clear band around 44 kDa is expected in samples expressing LHX5. Human, mouse, and rat brain lysates are suitable positive controls, given the established cross-reactivity.
• As a polyclonal antibody, it is advisable to titrate the antibody for optimal performance in your specific assay. A starting dilution range of 1:500–1:2000 for WB is typical; however, precise conditions must be determined empirically.
• For ELISA, pairing with a suitable detection antibody is recommended. Antigen coating concentrations and antibody titers should be optimized based on the experimental system.
• This antibody is not validated for applications beyond WB and ELISA; alternative uses may require additional validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, academic labs, and research institutes with a single-point sourcing solution for IVD raw materials. From bulk ancillary reagents to rare target antibodies like anti-LHX5, we streamline procurement across development stages. Partner with us to access a wide portfolio of validated monoclonal and polyclonal antibodies tailored for neuroscience and developmental biology applications.