Anti-LHX3 Monoclonal Antibody for WB, ELISA - Q9UBR4

Core Product Specifications and Parameters

Biological Background: LHX3 Function and Localization

• LHX3 (LIM/homeobox protein Lhx3) is a transcription factor containing two LIM domains and a homeobox DNA-binding domain, enabling sequence-specific DNA binding and transcriptional regulation.
• It recognizes and binds to the consensus motif 5'-AATTAATTA-3' in regulatory elements of target genes such as CGA and CHX10, positively modulating transcription, with co-activation by LDB2.
• It synergistically enhances transcription from the prolactin promoter in cooperation with POU1F1/Pit-1.
• LHX3 is required for the establishment of specialized cells of the pituitary gland and the nervous system, with a role in pituitary and neuronal development. Related references: PMID:21149718
• Involved in the development of interneurons and motor neurons in cooperation with LDB1 and ISL1.
• Subcellular localization is nuclear, consistent with its function as a transcription factor.
• Post-translational modifications include phosphorylation, and it is classified as a phosphoprotein; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within the C-terminal region (amino acids 250-350) of human LHX3, which includes part of the homeobox domain, potentially ensuring specific detection of LHX3 isoforms containing this region.
• For Western blotting, consider using whole-cell lysates from pituitary or neuronal cell lines, as LHX3 is a transcription factor expressed in these tissues.
• Validate antibody specificity in the relevant sample system, and consider peptide blocking experiments to confirm band identity.
• The antibody is validated for WB and ELISA; for other applications, titration and validation are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD antibodies and reagents. This anti-LHX3 antibody supports research in neurodevelopmental biology and pituitary function. For your sourcing needs, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assistance in obtaining raw materials for rare targets.