Anti-LHX1 Polyclonal Antibody for WB, ELISA - P48742

Core Product Specifications and Parameters

Biological Background: LHX1 Function and Localization

• LHX1, also known as Lim-1, is a LIM/homeobox protein that functions as a potential transcription factor.
• It contains LIM domains, which are zinc-binding motifs, and a homeobox DNA-binding domain, indicating its role in transcriptional regulation.
• LHX1 is implicated in early mesoderm formation and lateral mesoderm differentiation, as well as neurogenesis during embryonic development.
• The protein localizes to the nucleus, consistent with its function as a transcription factor.
• It is expressed in the brain, thymus, and tonsils; its expression has been detected in samples from chronic myeloid leukemia (CML) and acute myeloid leukemia (AML) cell lines.
• Keywords associated with LHX1 include developmental protein, differentiation, DNA-binding, neurogenesis, and phosphoprotein, reflecting its roles in development and signaling.

Experimental Guidance and Technical Tips

• For Western blot, consider using nuclear extracts or whole cell lysates from human, mouse, or rat cells/tissues, as LHX1 is a nuclear protein.
• The immunogen corresponds to amino acids 120–180, a region likely to be exposed; validate antibody specificity in your target species and sample type.
• For ELISA, recombinant LHX1 protein or peptide may be used as a standard to optimize coating and detection conditions.
• Since this is a polyclonal antibody, lot-to-lot variability may occur; always include appropriate positive and negative controls.
• Predicted molecular weight is approximately 45 kDa; confirm the observed band size in your system, as post-translational modifications may alter migration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies diagnostic manufacturers, labs, and research institutes with validated IVD raw materials, including a wide range of antibodies for transcription factors and developmental regulators like LHX1. Our one-stop platform offers optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your assay development from concept to clinic. For your LHX1-related projects, we provide reliable access to quality reagents and technical support, streamlining your procurement process.