Anti-LHX1 Polyclonal Antibody for WB and ELISA - P48742

Core Product Specifications and Parameters

Biological Background: LHX1 Function and Localization

• LHX1, also known as LIM1 or Lim-1, is a LIM/homeobox protein that functions as a potential transcription factor.
• It plays a role in early mesoderm formation, lateral mesoderm differentiation, and neurogenesis during embryonic development.
• The protein contains LIM domains and a homeobox domain, classifying it as a DNA-binding protein involved in transcription regulation and development.
• LHX1 localizes to the nucleus, consistent with its function in transcriptional control.
• It is expressed in the brain, thymus, and tonsils, and has been detected in samples from chronic myeloid leukemia (CML) and acute myeloid leukemia (AML) cell lines.
• Post-translational modifications of LHX1 include phosphorylation, and its LIM domains coordinate zinc ions, which are critical for protein structure and function.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminus of human LHX1; this region may be conserved in mouse and rat, consistent with the reported cross-reactivity.
• For Western blot (WB), anticipate a band near 45 kDa; use a positive control lysate expressing LHX1 to confirm specificity.
• In ELISA, consider coating with the immunogenic peptide or recombinant protein to optimize detection conditions.
• Since this is a polyclonal antibody, lot-to-lot variability may occur; validation in the relevant sample system is recommended.
• For mouse and rat samples, validate cross-reactivity experimentally due to potential species-specific differences.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers and research institutions as a comprehensive source for IVD raw materials. This anti-LHX1 antibody supports studies in developmental biology and transcription factor research. We offer bulk polyclonal antibodies, validated matched pairs, and customized sourcing for rare targets, facilitating seamless progression from research to clinical application.