Anti-LHPP Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H008

Core Product Specifications and Parameters

Biological Background: LHPP Function and Localization

• LHPP (Phospholysine phosphohistidine inorganic pyrophosphate phosphatase) is a hydrolase that specifically dephosphorylates phosphohistidine and phospholysine residues, regulating protein histidine phosphorylation signaling.
• It displays broad substrate specificity, also hydrolyzing inorganic diphosphate (PPi) and imidodiphosphate, albeit with lower catalytic efficiency.
• Localized in both the cytoplasm and nucleus, LHPP may participate in diverse cellular processes, including metabolic regulation and chromatin-associated functions.
• Tissue expression is highest in the brain, with lower levels detected in liver and kidney; thyroid expression is confirmed at the protein level, suggesting organ-specific roles.
• The enzyme requires magnesium or other divalent metal ions for activity, as indicated by metal-binding motifs and structural studies.
• Alternative splicing generates multiple LHPP isoforms, potentially contributing to functional diversity in different tissues or developmental stages.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the full-length protein (aa 1–270), so the antibody may recognize multiple isoforms and conformations; validate specificity with appropriate controls.
• For Western blotting, the predicted molecular weight is ~29 kDa; include molecular weight markers and consider phosphatase inhibitors during lysis to preserve target phosphorylation states.
• In immunofluorescence/ICC, optimize fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) conditions to detect nuclear and cytoplasmic pools.
• Cross-reactivity with mouse and rat enables translational studies; confirm optimal dilutions and sample compatibility in each species.
• Recombinant protein-based ELISA may be used for titration and specificity analysis; the antibody is suitable as a capture or detection reagent after validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with one-stop access to validated IVD raw materials, including polyclonal antibodies like this anti-LHPP antibody for metabolic and signaling research. Our comprehensive portfolio features optimized antibody pairs, bulk ancillary reagents, and rare-target raw-material sourcing, ensuring you can progress from assay concept to clinical application efficiently. Let CamelBio be your trusted partner in bridging supply gaps and accelerating diagnostic development.