Anti-LHCGR Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P22888

Core Product Specifications and Parameters

Biological Background: LHCGR Function and Localization

• LHCGR (Lutropin-choriogonadotropic hormone receptor), also known as Luteinizing hormone receptor (LHR, LSH-R), is a glycoprotein receptor belonging to the G protein-coupled receptor (GPCR) family, specifically the leucine-rich repeat-containing receptor subfamily.
• LHCGR functions as the receptor for lutropin (luteinizing hormone) and choriogonadotropic hormone, mediating signal transduction primarily through G protein activation of adenylate cyclase, which increases intracellular cAMP levels.
  Related references: PMID:11847099
• LHCGR is localized to the cell membrane, consistent with its role as a transmembrane receptor.
• The receptor is predominantly expressed in gonadal tissues (e.g., ovarian theca and granulosa cells, testicular Leydig cells) and thyroid cells, underlining its tissue-specific physiological roles.
• The LHCGR gene undergoes alternative splicing, generating multiple isoforms that may have distinct functional properties.
• Key structural features include an extracellular leucine-rich repeat domain, seven transmembrane helices, and intracellular regions subject to post-translational modifications such as palmitoylation and sulfation.
• Mutations in LHCGR are associated with various disorders, including precocious puberty and Leydig cell hypoplasia, highlighting its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen for this antibody spans amino acids 200–360 within the extracellular domain, which may be useful for detecting both full-length and soluble receptor forms. Consider validating detection of soluble LHCGR in your sample system.
• For Western blot (WB), the predicted molecular weight is 79 kDa; however, N-linked glycosylation may result in a higher apparent molecular weight. Include appropriate molecular weight markers and consider deglycosylation experiments to confirm specificity.
• For immunofluorescence (IF) on paraffin-embedded sections (IF-P), ensure optimal antigen retrieval and permeabilization to allow antibody access to the membrane-bound receptor.
• For ELISA applications, the antibody may be used as a capture or detection reagent in sandwich assays targeting the extracellular portion of LHCGR. Validate the assay with recombinant protein standards and relevant biological samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with a one-stop sourcing platform for IVD raw materials, including validated antibodies like this anti-LHCGR polyclonal antibody. As a dedicated supply chain partner, we offer bulk ancillary reagents, rare-target material sourcing, and technical support to streamline your development from concept to clinic. Leverage our LHCGR antibody for reproductive endocrinology research and next-generation diagnostic assay development.