Anti-LHCGR Polyclonal Antibody for WB, IF-P, ELISA - P22888

Core Product Specifications and Parameters

Biological Background: LHCGR Function and Localization

• LHCGR encodes the lutropin-choriogonadotropic hormone receptor, also known as luteinizing hormone receptor (LHR). The gene yields multiple isoforms via alternative splicing.
• The receptor mediates the actions of lutropin (LH) and choriogonadotropin (CG) through G protein-coupled signaling, activating adenylate cyclase (PubMed:11847099). Related references: PMID:11847099
• LHCGR is a member of the glycoprotein hormone receptor subfamily within the G protein-coupled receptor (GPCR) superfamily, characterized by a large extracellular domain containing leucine-rich repeats.
• The protein localizes to the cell membrane, consistent with its function as a transmembrane receptor.
• LHCGR is subject to several post-translational modifications, including N-linked glycosylation, palmitoylation, sulfation, and disulfide bond formation, which are critical for receptor function and stability.
• Tissue expression is primarily restricted to gonadal and thyroid cells, reflecting its central role in reproductive hormone signaling.
• Clinically, mutations in LHCGR are associated with disease variants, and the protein has been structurally characterized by 3D-structure determination.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide within amino acids 200–300 of human LHCGR (NP_000224.2). This region may be selected for conserved sequence characteristics, potentially supporting cross-reactivity with mouse LHCGR as indicated by the product specifications.
• For Western blot (WB), expected bands may be observed around the calculated molecular weight of 71–78 kDa based on the supplied protein weight; note that post-translational modifications can cause shifts.
• In immunofluorescence-paraffin (IF-P), consider appropriate permeabilization and antigen retrieval methods for membrane targets; validation in relevant gonadal or thyroid tissue samples is recommended.
• For ELISA applications, pairing with a suitable detection antibody and confirming linearity and sensitivity in the intended sample matrix (e.g., serum, plasma) is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies for targets like LHCGR, a key GPCR in reproductive endocrinology. Beyond individual reagents, we provide bulk ancillary reagents and facilitate rare target raw-material sourcing to support your assay development from concept to clinic. Let CamelBio be your trusted B2B partner in building robust, reproducible diagnostic solutions.