Anti-LH(CGA/LHB) Monoclonal Antibody for WB, IHC-P, ELISA - P01215 / P01229

Core Product Specifications and Parameters

Biological Background: LH(CGA/LHB) Function and Localization

• The CGA protein is the common alpha subunit shared by four anterior pituitary glycoprotein hormones: thyroid-stimulating hormone (TSH), luteinizing hormone (LH), follicle-stimulating hormone (FSH), and human chorionic gonadotropin (CG).
• It forms non-covalently linked heterodimers with distinct beta subunits (e.g., LHB for LH) to produce biologically active hormones that bind specific cell-surface receptors on target cells.
• The alpha subunit is synthesized as a precursor with a signal peptide, which is cleaved to generate the mature secreted protein; it contains several conserved disulfide bonds and N-glycosylation sites critical for proper folding and hormone activity.
• According to UniProt, CGA is a secreted glycoprotein with known 3D structures and has been characterized by direct protein sequencing, confirming its role in endocrine regulation.

Experimental Guidance and Technical Tips

• The immunogen was designed using the mature peptide regions (aa 25-116) of both CGA and LHB, which excludes the signal peptide; this region covers the functional domains of the subunits.
• For Western blotting under reducing conditions, expect bands at approximately 13 kDa (CGA) and 15 kDa (LHB); consider using positive controls such as pituitary extract or recombinant heterodimer preparations.
• For immunohistochemistry (IHC-P), antigen retrieval (e.g., heat-induced epitope retrieval at pH 6 or 9) may be required to unmask the epitope; validate in relevant tissue samples known to express LH, such as pituitary or placental tissues.
• The antibody is applicable to ELISA; when setting up sandwich ELISA, pair with another antibody recognizing a distinct epitope on the same subunit or the cognate beta subunit to avoid steric hindrance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, purified antigens, and ancillary reagents for reproductive hormone assay development. The LH(CGA/LHB) rabbit monoclonal antibody fits into a portfolio supporting thyroid/reproductive function testing, and we can assist with bulk orders or custom sourcing of rare targets.