Anti-LHB Rabbit Monoclonal Antibody for IF-P, IHC-P, ELISA - P01229

Core Product Specifications and Parameters

Biological Background: LHB Function and Localization

• LHB encodes the beta subunit of luteinizing hormone (LH), a gonadotropin glycoprotein hormone essential for reproductive function.
• LH is synthesized in and secreted from the anterior pituitary gland, acting on the gonads to promote steroidogenesis, spermatogenesis in males, and ovulation in females.
• The beta subunit confers biological specificity, forming a heterodimer with the common alpha subunit (CGA) to constitute intact LH.
• LHB is a secreted protein with a signal peptide and contains multiple disulfide bonds critical for structural integrity and bioactivity.
• Mutations in LHB are associated with hypogonadotropic hypogonadism, a condition characterized by delayed puberty and infertility.
• The protein undergoes glycosylation, a post-translational modification important for proper folding, secretion, and receptor binding.
• LHB is classified as a reference proteome member and has been identified through direct protein sequencing and proteomics analysis.

Experimental Guidance and Technical Tips

• The immunogen (a.a. 21–141 of human LHB) covers the mature peptide; this design may target both free LHB and the LH heterodimer, so careful validation in the intended assay format is advised.
• For IHC-P, human pituitary tissue serves as a positive control; optimize antigen retrieval and antibody dilution to achieve clear staining of gonadotroph cells.
• In IF-P, signal is expected in the cytoplasm and potentially in secretory vesicles; include a secretory pathway marker or co-staining with CGA to confirm specificity.
• Given that LHB is secreted, fixation and permeabilization steps must be rigorously tested to detect both intracellular and extracellular forms.
• For ELISA, the antibody can be evaluated as a capture or detection reagent; cross-reactivity with highly homologous beta subunits (e.g., CGB) should be experimentally assessed.

CamelBio: Your One-Stop Sourcing Bridge

As your dedicated one-stop IVD raw-material partner, CamelBio supports diagnostic developers and researchers with high-quality antibodies, antigen pairs, and ancillary reagents for reproductive hormone assays. We offer validated monoclonal antibodies like this anti-LHB rabbit mAb, alongside bulk sourcing, technical consultation, and custom development services to accelerate your assay pipeline from discovery to clinical application. From core reagents to rare targets, CamelBio ensures reliable supply and expert support for your endocrinology-focused diagnostic projects.