Anti-LGR6 Rabbit Polyclonal Antibody for WB and ELISA - Q9HBX8

Core Product Specifications and Parameters

Biological Background: LGR6 Function and Localization

• LGR6 (Leucine-rich repeat-containing G protein-coupled receptor 6) is encoded by the LGR6 gene and is also known as GPCR or VTS20631. It belongs to the glycoprotein hormone receptor subfamily of GPCRs.
• Acts as a receptor for R-spondins (RSPO1, RSPO2, RSPO3, RSPO4), potentiating the canonical Wnt signaling pathway by associating with phosphorylated LRP6 and frizzled receptors upon ligand binding.
• In contrast to classical GPCRs, LGR6 does not activate heterotrimeric G proteins; instead, it serves as a marker of multipotent stem cells in the epidermis and may function as a tumor suppressor.
• Predominantly localized to the cell membrane, consistent with its role as a transmembrane receptor involved in signal transduction.
• Structural hallmarks include leucine-rich repeats, disulfide bonds, and glycosylation, which are characteristic of the LGR family and essential for ligand interaction and receptor stability.
• The protein undergoes alternative splicing, producing multiple isoforms that may have tissue-specific or context-dependent functions.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight of LGR6 is approximately 104 kDa. Consider using denaturing and reducing conditions along with appropriate molecular weight markers and a suitable loading control.
• The immunogen corresponds to amino acids 350–510, a region that may be partially intracellular or extracellular; the antibody could detect multiple isoforms or processed forms. Validate specificity in your experimental system.
• In ELISA, this polyclonal antibody can be employed for target capture or detection. Optimize coating concentrations and consider pairing with monoclonal antibodies when developing a sandwich assay.
• The antibody demonstrates cross-reactivity with mouse and rat LGR6, facilitating translational studies in rodent models; confirm reactivity in the relevant tissue or cell line.
• As a polyclonal preparation, include both positive and negative controls in each experiment to monitor batch-to-batch consistency and assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing service for IVD raw materials, including validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents tailored to your assay development needs. For LGR6-related research in stem cell biology, Wnt signaling, and oncology, we facilitate access to high-quality reagents and technical support to accelerate your project from concept to clinic. Contact us for customized raw material procurement and consulting.