Anti-LGR5/GPR49 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O75473

Core Product Specifications and Parameters

Biological Background: LGR5 Function and Localization

• Leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5), also known as G protein-coupled receptor 49 (GPR49), GPR67, and HG38, is encoded by the LGR5 gene.
• LGR5 functions as a receptor for R-spondins (RSPO1-4) and potentiates the canonical Wnt signaling pathway, serving as a stem cell marker for intestinal epithelium and hair follicles.
• Upon R-spondin binding, LGR5 associates with phosphorylated LRP6 and frizzled receptors, activating Wnt target gene expression without activating heterotrimeric G proteins, distinguishing it from classical GPCRs.
• LGR5 is localized to the cell membrane and the trans-Golgi network membrane, with roles in stem cell maintenance during postembryonic development.
• The protein contains leucine-rich repeats and is subject to post-translational modifications including glycosylation and disulfide bond formation.
• Tissue expression includes skeletal muscle, placenta, spinal cord, and brain; it is specifically expressed in the stem cell compartment at the base of intestinal crypts. Overexpression is observed in ovarian, colon, and liver cancers.
• Alternative splicing generates multiple isoforms, and the protein has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 824-907 of human LGR5, a C-terminal region; the antibody may recognize full-length LGR5.
• For western blotting, the expected band is approximately 100 kDa; validate with positive control lysates from LGR5-expressing cells or tissues.
• In immunohistochemistry-paraffin (IHC-P), consider standard antigen retrieval protocols and validate using intestinal crypt sections as positive control.
• The antibody is reported to cross-react with mouse and rat, suggesting conserved epitope; confirm specificity with appropriate controls in these species.
• As a stem cell marker, LGR5 expression may be low; signal amplification or enrichment strategies can be explored if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to IVD raw materials, technical services, and consulting. This anti-LGR5 antibody supports stem cell and oncology research, and we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare target sourcing, our team can assist from concept to clinic.