Anti-LGR5/GPR49 Monoclonal Antibody for WB and ELISA - O75473

Core Product Specifications and Parameters

Biological Background: LGR5/GPR49 Function and Localization

• LGR5 (Leucine-rich repeat-containing G protein-coupled receptor 5) is also known as GPR49, GPR67, and HG38, and belongs to the G protein-coupled receptor family with leucine-rich repeats in its extracellular domain.
• It functions as a receptor for R-spondins (RSPO1-4), potentiating the canonical Wnt signaling pathway by associating with phosphorylated LRP6 and frizzled receptors.
• Unlike classical GPCRs, LGR5 does not activate heterotrimeric G proteins to transduce signals; instead, it acts as a stem cell marker in the intestinal epithelium and hair follicle.
• The protein is localized to the cell membrane, Golgi apparatus, and trans-Golgi network membrane.
• LGR5 undergoes alternative splicing, glycosylation, and contains disulfide bonds, as indicated by its UniProt keywords.
• It is expressed in skeletal muscle, placenta, spinal cord, and brain, and is specifically found at the base of crypts in colonic and small intestinal stem cells.
• LGR5 is overexpressed in several cancers, including ovarian, colon, and liver cancers, and its expression pattern shifts in premalignant lesions.

Experimental Guidance and Technical Tips

• The immunogen was designed from a synthetic peptide corresponding to amino acids 800-907 of human LGR5/GPR49 (O75473), targeting a region within the intracellular domain; this antibody is expected to detect total LGR5.
• For Western blotting, a band around 100 kDa is predicted; validate the signal in relevant positive controls such as intestinal organoids or LGR5-expressing cancer cell lines.
• For ELISA, consider pairing this antibody with a detection antibody against a non-overlapping epitope to build a sensitive sandwich assay.
• Cross-reactivity to mouse and rat was observed; thus, this antibody can be used for studies in these species, but validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to high-quality IVD raw materials, including validated antibodies for stem cell and cancer research like this anti-LGR5/GPR49 monoclonal antibody. Our portfolio encompasses optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing solutions, supporting your assay development from concept to clinic.