Anti-LGR5 Polyclonal Antibody for WB, FC, ELISA - O75473

Core Product Specifications and Parameters

Biological Background: LGR5 Function and Localization

• LGR5 (Leucine-rich repeat-containing G protein-coupled receptor 5), also referred to as GPR49, GPR67, HG38, FEX, or GRP49, is a multi-pass membrane receptor characterized by leucine-rich repeats and a 7-transmembrane domain.
• It functions as a receptor for R-spondins (RSPO1–4), potentiating canonical Wnt signaling. Upon ligand binding, LGR5 associates with phosphorylated LRP6 and Frizzled receptors to activate Wnt target gene expression, without engaging heterotrimeric G proteins.
• LGR5 is a well-established marker for adult stem cells, particularly in the intestinal crypt base and the hair follicle bulge, and is essential for the maintenance and development of these epithelial stem cell populations.
• Subcellularly, LGR5 localizes to the cell membrane, Golgi apparatus, and trans-Golgi network membrane.
• Tissue expression includes skeletal muscle, placenta, spinal cord, and various brain regions. In the gastrointestinal tract, LGR5 is specifically expressed in stem cells at the crypt base of colonic and small intestinal mucosa. In premalignant conditions, its expression extends beyond the crypt base, and it is overexpressed in ovarian, colon, and liver cancers.
• Post-translational modifications include disulfide bonds and glycosylation. Alternative splicing generates multiple isoforms, and the protein contains a signal peptide for secretion pathway targeting.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide from the C‑terminal region (aa 480–580) of human LGR5. In Western blot (WB), consider verifying detection of the full-length protein at the predicted molecular weight of ~100 kDa (calculated 99,998 Da) under reducing conditions.
• For flow cytometry (FC), LGR5 is a cell‑surface receptor; use intact, non‑permeabilized cells to detect surface expression. Confirm staining in cell lines or primary tissues with known LGR5 expression (e.g., intestinal organoids) and include isotype controls.
• In ELISA applications, this polyclonal antibody can serve as a capture or detection reagent. Cross‑reactivity with mouse LGR5 is reported; however, validation in the specific assay format is recommended.
• Peptide blocking or knockout cell lines can be used as specificity controls to distinguish specific from non‑specific signals in all applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with reliable access to high‑quality IVD raw materials like this anti‑LGR5 polyclonal antibody, which supports research into intestinal stem cell biology and cancer. We offer a comprehensive portfolio including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare‑target raw materials. Combined with technical services and consulting, CamelBio serves as your end‑to‑end partner from concept to clinic.