Anti-LGI1 Rabbit Monoclonal Antibody for WB and ELISA - O95970

Core Product Specifications and Parameters

Biological Background: LGI1 Function and Localization

• Leucine-rich glioma-inactivated protein 1 (LGI1), also known as epitempin-1, is a secreted protein that belongs to the LGI family characterized by leucine-rich repeats (LRRs). It is encoded by the LGI1 gene (Gene ID: 9211) and undergoes alternative splicing to produce multiple isoforms.

• LGI1 plays a critical role in regulating voltage-gated potassium channels by slowing their inactivation, specifically those composed of KCNA1, KCNA4, and KCNAB1 subunits. It acts by preventing KCNAB1-mediated channel closure, thus modulating neuronal excitability.

• As a ligand for the postsynaptic receptor ADAM22, LGI1 positively regulates synaptic transmission mediated by AMPA-type glutamate receptors, contributing to synaptic plasticity and cognitive processes.

• LGI1 suppresses the production of matrix metalloproteinases MMP1 and MMP3 through the phosphatidylinositol 3-kinase/ERK signaling pathway, indicating a role in extracellular matrix remodeling and neuroinflammation. Related references: PMID:15047712

• Subcellularly, LGI1 is found in multiple compartments including the synapse, cytoplasm, Golgi apparatus, and endoplasmic reticulum, and it is also secreted into the extracellular space, consistent with its dual roles in synaptic function and extracellular signaling.

• The protein is post-translationally modified by glycosylation (as indicated by its glycoprotein keyword) and features leucine-rich repeat domains, which are common in protein-protein interaction modules.

• Tissue-specific expression analysis reveals high abundance in the temporal neocortex, with lower levels in other cortical regions and hippocampus, and absence in the cerebellum. This regional distribution aligns with its critical roles in cortical network function and epileptogenesis. Related references: PMID:16787412

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was raised against a synthetic peptide immunogen mapping to amino acids 150-250 of human LGI1. The immunogen region lies within the extracellular domain, suggesting suitability for detecting secreted and membrane-associated forms of the protein in denaturing and native conditions (WB and ELISA).
• For Western blot, consider optimizing protein extraction methods to account for LGI1's secretion and variable subcellular localization; sample preparation may influence detection sensitivity.
• In ELISA applications, the antibody can be used as a capture or detection reagent; validate specificity using recombinant LGI1 or known positive tissue lysates.
• Given the reported cross-reactivity with mouse and rat, this antibody is a candidate tool for translational studies, but endogenous expression patterns should be verified in the relevant model systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive sourcing of IVD raw materials, including validated monoclonal antibodies like this anti-LGI1 clone. We support neuroscience and synaptic research by offering customized antibody pairs, bulk ancillary reagents, and logistics for rare targets. For LGI1 studies, our team facilitates access to quality-controlled reagents from antibody characterization to purification, ensuring a smooth transition from concept to clinical application.