Anti-LGI1 Polyclonal Antibody for WB, ELISA - O95970

Core Product Specifications and Parameters

Biological Background: LGI1 Function and Localization

• LGI1, or epitempin-1, is a secreted glycoprotein belonging to the leucine-rich repeat (LRR) superfamily. It is encoded by the LGI1 gene in humans, with a calculated molecular mass of approximately 63.8 kDa (UniProt: O95970).
• It functions as a regulator of voltage-gated potassium (Kv) channels, specifically slowing the inactivation of channels composed of KCNA1, KCNA4, and KCNAB1 subunits by preventing KCNAB1-mediated channel closure (By similarity).
• LGI1 acts as a ligand for the transmembrane protein ADAM22, positively regulating synaptic transmission mediated by AMPA-type glutamate receptors (By similarity).
• Through the phosphatidylinositol 3-kinase/ERK signaling pathway, LGI1 suppresses the production of matrix metalloproteinases MMP1 and MMP3, indicating a role in extracellular matrix remodeling (PubMed:15047712). Related references: PMID:15047712
• Subcellularly, LGI1 is present in the cytoplasm, endoplasmic reticulum, Golgi apparatus, and is also secreted and localized to synapses, consistent with its roles in both intracellular trafficking and intercellular signaling.
• Tissue expression is predominantly neural, with highest levels in the temporal neocortex, parietal and frontal cortices, and putamen; lower in occipital cortex and hippocampus; and absent in the cerebellum (at protein level) (PubMed:16787412). Related references: PMID:16787412
• Post-translational modifications include N-linked glycosylation, and alternative splicing generates multiple transcript variants, though their functional distinctions are not well characterized.
• Mutations in LGI1 are associated with autosomal dominant lateral temporal lobe epilepsy (ADLTE) and intellectual disability, highlighting its critical role in neuronal function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 301-557 of human LGI1 (C-terminal region). This antibody is expected to recognize the C-terminal portion of the protein, potentially detecting both full-length and some truncated isoforms containing this region.
• For Western blot analysis, a band at approximately 64 kDa is anticipated. It is advisable to validate molecular weight with a positive control lysate from human, mouse, or rat brain tissue, as cross-reactivity is reported for all three species.
• For ELISA, the antibody can be used for direct or indirect detection of recombinant or native LGI1. Optimal working concentrations should be determined empirically.
• Upon receipt, store the antibody at -20°C or as indicated on the vial for long-term stability, avoiding repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive procurement partner for IVD raw materials, offering a wide range of antibodies, antigens, and ancillary reagents to support diagnostic and life science research workflows. For neuroscience targets like LGI1, we provide validated rabbit polyclonal antibodies suitable for WB and ELISA, along with bulk sourcing options for large-scale studies. Our team can also assist in sourcing rare and custom raw materials for single- or multiplexed assay development, streamlining your path from concept to clinic.