Anti-LGALS3BP Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q08380

Core Product Specifications and Parameters

Biological Background: Galectin-3-binding protein Function and Localization

• Galectin-3-binding protein (LGALS3BP), also known as 90K, Mac-2-binding protein, and tumor-associated antigen 90K, is a secreted glycoprotein encoded by the LGALS3BP gene in humans.
• It promotes integrin-mediated cell adhesion and may play a role in host defense against viruses and tumor cells (Keywords: Cell adhesion).
• The protein is secreted and localizes to the extracellular space and extracellular matrix (UniProt Q08380).
• LGALS3BP is ubiquitously expressed and detected in various body fluids including semen, milk, serum, tears, saliva, and urine, as well as expressed by keratinocytes and fibroblasts.
• It contains disulfide bonds and is a glycoprotein, with a molecular weight of approximately 65 kDa (UniProt: 65331 Da).
• Additional structural features include a signal peptide (required for secretion), and it is a target of proteomics identification, as annotated in the UniProt reference proteome.
• The protein may function as a basement membrane autoantigen p105, highlighting its role in extracellular matrix organization.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 306–585 of human LGALS3BP (C‑terminal region), which may allow detection of full-length and certain processed forms; consider validating in your sample system.
• This antibody is suitable for WB, IF/ICC, and ELISA. For WB and ELISA, the predicted band size is ~65 kDa; verify band specificity with appropriate controls.
• Cross-reactivity is listed for human, mouse, and rat; optimization of dilution and blocking conditions is advised for each species and application.
• Due to the ubiquitous expression and presence in body fluids, include relevant negative controls and consider sample preparation techniques to minimize background.
• The antibody has been validated using recombinant antigen; endogenous detection may require additional optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials and technical services. This anti-LGALS3BP polyclonal antibody is offered as part of our comprehensive catalog covering cancer, cell adhesion, and immunology targets. In addition to validated antibody pairs and optimized monoclonal/polyclonal antibodies, we supply bulk ancillary reagents and can assist with rare target raw-material sourcing to streamline your development pipeline.