Anti-LF1 Rabbit Polyclonal Antibody for WB, ELISA - Q6TAQ6

Core Product Specifications and Parameters

Biological Background: LF1 (Homeobox-leucine zipper protein HOX10) Function and Localization

• LF1, designated Homeobox-leucine zipper protein HOX10, is a homeodomain transcription factor belonging to the HD-ZIP protein family.
• It is also known as OsHB1 and OsHox10, encoded by the HOX10 (HB1) gene.
• The protein likely functions as a transcription factor, regulating gene expression through DNA binding.
• LF1 localizes to the nucleus, consistent with its role in transcriptional regulation.
• Expression is detected in stems, leaf sheaths, leaf blades, and panicles, suggesting involvement in plant development.
• Key structural features include a homeobox DNA-binding domain and a leucine zipper motif (coiled coil), which may mediate dimerization.
• The protein has a calculated molecular weight of approximately 92 kDa (91829 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 400-500 of LF1; thus, the antibody may recognize this region. For WB, consider using a low-percentage SDS-PAGE gel and extended transfer times to handle the ~92 kDa protein.
• Positive expression has been reported in stems, leaf sheaths, blades, and panicles; use extracts from these tissues as positive controls.
• For ELISA, pair this polyclonal antibody with a monoclonal detection antibody against LF1 for a sandwich format; however, batch-to-batch variability is common with polyclonals, so validate consistency.
• Cross-reactivity is expected with Oryza sativa; for other species, test beforehand if targeting orthologs.
• Since LF1 is a transcription factor, nuclear enrichment may improve WB signal; consider subcellular fractionation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a comprehensive one-stop platform for IVD raw material sourcing. This LF1 rabbit polyclonal antibody supports plant biology and transcription factor research, and we can also provide validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Our sourcing capabilities extend to rare targets like plant-specific transcription factors, helping streamline your development from concept to clinic.