Anti-Leukosialin (SPN/CD43) Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P16150

Core Product Specifications and Parameters

Biological Background: Leukosialin Function and Localization

• Leukosialin (SPN/CD43) is a predominant cell surface sialoprotein expressed on leukocytes, including thymocytes, T-lymphocytes, neutrophils, plasma cells, and myelomas.
• Regulates multiple T-cell functions such as activation, proliferation, differentiation, trafficking, and migration; facilitates lymph node homing via association with ERM proteins (EZR, RDX, MSN).
• Negatively regulates Th2 cell differentiation, promoting Th1 lineage commitment, and induces IFN-gamma expression in T-cells during TCR activation. Related references: PMID:18036228
• Prepares T-cells for cytokine sensing by inducing expression of IFNGR and IL4R, enhancing their signaling, and clustering IFNGR with TCR. Related references: PMID:24328034
• Functions as a major E-selectin ligand mediating Th17 cell rolling on activated vasculature during inflammation; also acts as a T-cell counter-receptor for SIGLEC1.
• Provides anti-apoptotic signals that promote cell survival.
• Localizes to the cell membrane, microvilli, uropodium, and also to the nucleus and PML bodies.
• Extensively modified by glycosylation and phosphorylation; identified as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 276-400) and may recognize both glycosylated and unglycosylated forms; consider this when interpreting band shifts.
• For Western blotting, a band near 40 kDa is expected, though extensive glycosylation can cause an apparent higher molecular weight. Validate in the relevant sample system.
• Optimize antibody dilutions for IHC-P and IF/ICC applications; antigen retrieval conditions should be tested empirically.
• Confirm reactivity in mouse and rat samples due to reported cross-reactivity; validate in the target tissue/cell type.

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CamelBio connects diagnostic manufacturers and research laboratories with high-quality IVD raw materials such as validated antibodies for leukocyte biology. Whether you need polyclonal antibodies like anti-SPN for T-cell studies, bulk ancillary reagents, or support in rare-target sourcing, our platform provides a streamlined procurement channel from discovery to clinical applications.