Anti-LETMD1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q6P1Q0

Core Product Specifications and Parameters

Biological Background: LETMD1 Function and Localization

• LETMD1 (LETM1 domain-containing protein 1) is also known as cervical cancer proto-oncogene 1 protein p40 (HCCR-1), HCCR2, and SLC55A3. It is a multi-pass membrane protein belonging to the LETM1 family.

• Plays an essential role in mitochondrial structure and function, and is required for thermogenesis of brown adipocytes. In brown adipose tissue, it localizes to the nucleus and interacts with chromatin remodeler SMARCA4 to regulate thermogenic genes such as UCP1 (By similarity).

• Involved in the regulation of phagocytosis and inflammatory responses to lipopolysaccharide (LPS) in macrophages. Related references: PMID:31980577

• Functions in tumorigenesis and may act as a negative regulator of the p53/TP53 tumor suppressor pathway. Related references: PMID:12879013

• Subcellularly localized to the mitochondrial outer membrane, inner membrane, and nucleus.

• Expressed in kidney, liver, skeletal muscle, heart, and brain. Overexpressed in various tumors, including leukemia, lymphoma, and carcinomas of the breast, kidney, ovary, stomach, colon, and uterine cervix.

• Keywords associated with LETMD1 include alternative splicing, mitochondrion, nucleus, proto-oncogene, and transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 161–360 of human LETMD1) targets a central region of the protein, which may detect multiple isoforms and be suitable for applications such as WB, IHC, IF, and ELISA.

• For Western blot, consider using a starting dilution of 1:500–1:2000 and validate with appropriate positive and negative controls. Endogenous LETMD1 expression may vary across tissues; use a high-expression tissue (e.g., kidney or liver) as positive control.

• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimization of antigen retrieval, fixation, and permeabilization is recommended. Include isotype controls to assess specificity.

• Cross-reactivity is reported for human, mouse, and rat; if using in other species, sequence homology analysis of the immunogen region is advised.

• ELISA can be used for antibody titer determination or antigen detection, but pairing with a matched capture antibody may be required for sandwich ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. For rare targets like LETMD1, our team leverages global supplier networks to secure high-quality raw materials, ensuring reliable supply for your mitochondrial and oncology research applications.