Anti-LETM1 Rabbit Polyclonal Antibody for WB and ELISA - O95202

Core Product Specifications and Parameters

Biological Background: LETM1 Function and Localization

• LETM1 (Leucine zipper-EF-hand-containing transmembrane protein 1), also known as Mdm38, SLC55A1, and electroneutral mitochondrial K+/H+ exchanger (KHE), is a mitochondrial inner membrane protein with a molecular weight of approximately 83 kDa.
• It functions as a mitochondrial proton/calcium antiporter, mediating proton-dependent calcium efflux from mitochondria (PubMed:19797662, PubMed:24344246, PubMed:29123128). Related references: PMID:19797662 PMID:24344246 PMID:29123128
• LETM1 also acts as an electroneutral mitochondrial proton/potassium exchanger, contributing to potassium homeostasis (PubMed:24898248, PubMed:36055214, PubMed:36321428). Related references: PMID:24898248 PMID:36055214 PMID:36321428
• It plays a crucial role in maintaining mitochondrial morphology by preserving tubular networks and cristae organization (PubMed:18628306, PubMed:32139798). Related references: PMID:18628306 PMID:32139798
• LETM1 is required for the assembly of respiratory chain supercomplexes (PubMed:18628306, PubMed:36055214). Related references: PMID:18628306 PMID:36055214
• Keywords from UniProt indicate involvement in calcium and potassium transport, and link to neurodegeneration, with evidence of disease variants.
• Post-translational modifications include acetylation and phosphorylation, and multiple isoforms exist due to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1–140 amino acids of human LETM1; thus, the antibody is expected to recognize epitopes in this region. In WB, a reducing SDS-PAGE is recommended to detect the full-length protein at ~83 kDa.
• Cross-reactivity with mouse and rat suggests suitability for studies in rodent models; however, validation in the relevant species and tissue type is advised.
• For ELISA, consider using the recombinant LETM1 protein as a positive control to establish assay conditions. Optimize coating concentrations and blocking conditions based on your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering this anti-LETM1 antibody to support mitochondrial biology and neurodegeneration research. We specialize in supplying validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents, ensuring seamless procurement for diagnostic manufacturers and research institutes.