Anti-Leptin Receptor Polyclonal Antibody for WB, IF/ICC, ELISA - P48357

Core Product Specifications and Parameters

Biological Background: Leptin Receptor Function and Localization

• The LEPR gene encodes the leptin receptor (OB-R, CD295, HuB219), a single-pass transmembrane protein belonging to the class I cytokine receptor family. Multiple isoforms (e.g., A, B) arise via alternative splicing, exhibiting distinct tissue distributions and functional roles.

• As the high-affinity receptor for the hormone leptin, LEPR mediates central and peripheral effects through activation of signaling cascades such as JAK2/STAT3 and MAPK/FOS. In the hypothalamus, it regulates appetite, energy expenditure, and body weight by modulating neuropeptide expression (e.g., POMC, AgRP). Related references: PMID:9537324

• The receptor is predominantly localized to the cell membrane and basolateral membrane, with a secreted form also detected. It plays a critical role in energy homeostasis, bone mass regulation, reproductive function, and glucose metabolism.

• In peripheral tissues, LEPR influences basal metabolism, insulin secretion, angiogenesis, and immune responses. It differentially regulates naive and memory T-cell proliferation and promotes a Th1 cytokine profile. Related references: PMID:12504075, PMID:25060689, PMID:8805376

• Isoform A is widely expressed in fetal liver, hematopoietic tissues, and adult heart, liver, small intestine, prostate, and ovary. Isoform B is highly expressed in the hypothalamus and skeletal muscle. Both isoforms are present in gastric mucosal cells and NK cells. Related references: PMID:19159218, PMID:12504075

• Post-translational modifications include N-glycosylation, phosphorylation, and disulfide bond formation, which may influence receptor stability, trafficking, and signaling capacity.

• A short isoform may function to transport leptin across the blood-brain barrier or act as a decoy receptor, antagonizing the full-length receptor isoforms. Related references: PMID:22405007

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1070–1165 within the intracellular domain; consequently, the antibody is expected to recognize total LEPR regardless of ligand-binding status or ectodomain shedding.
• The predicted molecular weight is approximately 132 kDa, but multiple bands or a broader signal may appear due to glycosylation and isoform diversity. Validate detection in the relevant sample system using appropriate controls.
• For Western blotting, consider using reducing conditions to dissociate disulfide-linked complexes.
• For immunofluorescence (IF/ICC), optimize permeabilization conditions to ensure accessibility of the intracellular epitope.
• For ELISA, confirm compatibility as either a capture or detection reagent in your specific buffer and matrix conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. For leptin receptor research—spanning metabolic, obesity, and immuno-oncology applications—we supply validated antibodies, matched detection pairs, and ancillary reagents at bulk and custom scales. Our global network also streamlines access to rare targets, enabling accelerated assay development without compromising supply continuity.