Anti-Leptin Receptor Monoclonal Antibody for WB, IHC-P, ELISA - P48357

Core Product Specifications and Parameters

Biological Background: Leptin Receptor Function and Localization

• Leptin receptor (LEPR) is a single-pass transmembrane receptor of the class I cytokine receptor family; multiple isoforms arise from alternative splicing, including the long signaling‑competent isoform B (Ob-Rb) and shorter isoforms with transport or regulatory roles.
• Isoform B acts as the primary receptor for the hormone leptin, mediating its effects via JAK2/STAT3 and MAPK/FOS signaling cascades; in the hypothalamus, it reduces food intake, increases energy expenditure, and regulates bone mass and hypothalamo-pituitary-adrenal hormone secretion. Related references: PMID:9537324
• In peripheral tissues, leptin receptor activation enhances basal metabolism, influences reproductive function, regulates pancreatic β‑cell function and insulin secretion, and modulates innate and adaptive immunity. Related references: PMID:12504075, PMID:25060689, PMID:8805376
• Certain short isoforms, such as isoform A, may facilitate leptin transport across the blood‑brain barrier and mediate leptin endocytosis without triggering STAT3 phosphorylation.
• A soluble isoform of LEPR antagonizes leptin binding and endocytosis by membrane‑bound isoforms A and B, acting as a regulatory decoy.
• The receptor localizes predominantly to the cell membrane and basolateral membrane, while a secreted isoform exists in extracellular fluids.
• Isoform B is highly expressed in the hypothalamus and skeletal muscle; isoform A is found in fetal liver, hematopoietic tissues, adult heart, liver, small intestine, prostate, ovary, and gastric mucosal epithelium.
• Post‑translational modifications, including glycosylation, phosphorylation, and disulfide bond formation, are critical for receptor folding, stability, and signaling competence.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1000–1100 within the cytoplasmic domain; for intracellular epitope detection, consider including a permeabilization step in staining protocols.
• By Western blot, the full‑length receptor migrates at approximately 132 kDa; ensure adequate transfer conditions and appropriate molecular weight markers for high‑molecular‑weight proteins.
• For immunohistochemistry (paraffin‑embedded sections), antigen retrieval may be required; optimize primary antibody dilution and detection system for your tissue type.
• Reported cross‑reactivity with mouse and rat suggests a conserved epitope; validate the antibody in your target species’ tissue or cell line using appropriate controls.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For studies on obesity, appetite regulation, or metabolic signaling, our one‑stop sourcing model streamlines procurement of rare‑target reagents such as leptin receptor antibodies and associated supplies. Reach out to explore how CamelBio can support your assay development and raw‑material needs.