Anti-Leptin Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P41159

Core Product Specifications and Parameters

Biological Background: Leptin Function and Localization

• Leptin (OB, OBS) is a key hormone in energy homeostasis, regulating body weight by binding to the leptin receptor (LEPR) and activating JAK2-STAT3, MAPK, and PI3K-AKT signaling pathways. Related references: PMID:15899045, PMID:17344214, PMID:19688109
• In the hypothalamus, leptin suppresses appetite by acting on POMC and NPY neurons, thereby reducing food intake and increasing energy expenditure; it also regulates bone mass and the hypothalamic-pituitary-adrenal axis.
• Peripheral roles include increasing basal metabolism, modulating pancreatic β-cell function and insulin secretion, and exerting pro-angiogenic effects on endothelial cells. Related references: PMID:11460888, PMID:19688109, PMID:24340098
• Leptin reduces glucose absorption in the intestine by activating PKC and downstream p38, PI3K, and ERK pathways. Related references: PMID:24340098
• It acts as a growth factor on various tissues, upregulating CCND1 and VEGFA via JAK2-STAT3 and MAPK/PI3K pathways, and may also promote apoptosis through BIRC5. Related references: PMID:17344214, PMID:18242580
• Leptin modulates both innate and adaptive immunity: it enhances neutrophil chemotaxis and phagocytosis, promotes pro-inflammatory cytokine secretion, and influences T-cell proliferation and differentiation. Related references: PMID:12504075, PMID:15899045, PMID:19688109, PMID:25060689
• The protein is secreted primarily by adipose tissue, with additional production in skeletal muscle, salivary gland ducts, and gastric mucosa. Related references: PMID:12448771, PMID:16052473, PMID:7789654

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N-terminal region (aa 1-100); this may allow detection of both full-length and potentially processed forms of leptin.
• For Western blot (WB), a band at approximately 19 kDa is expected; validate in leptin-expressing tissues such as adipose tissue or appropriate cell lines.
• For immunofluorescence/immunocytochemistry (IF/ICC), consider cell permeabilization to detect intracellular leptin pools.
• For ELISA, the antibody can be utilized for capture or detection; pairing with a monoclonal antibody may improve specificity and sensitivity. Validate in serum or culture supernatants.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, research laboratories, and institutes from concept to clinic. For leptin and metabolic disease research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. Our technical services and consulting help streamline the development of robust assays for obesity, diabetes, and energy metabolism studies.