Anti-Leptin Rabbit Polyclonal Antibody for IHC-P, ELISA - P41159

Core Product Specifications and Parameters

Biological Background: Leptin Function and Localization

• Leptin (also known as obese protein or obesity factor) is a secreted adipokine predominantly produced by adipose tissue, but also by skeletal muscle, salivary glands, and gastric mucosa, with roles in energy homeostasis and immune modulation.
• In the hypothalamus, Leptin acts via LEPR to suppress appetite by activating POMC neurons and inhibiting NPY neurons, thereby reducing food intake and increasing energy expenditure. Related references: PMID:15899045 PMID:17344214 PMID:19688109
• Peripherally, Leptin regulates pancreatic β-cell function, insulin secretion, and glucose absorption in the intestine through PKC-p38-PI3K-ERK signaling, highlighting its role in systemic metabolism. Related references: PMID:24340098
• Leptin exhibits pro-angiogenic and mitogenic effects on vascular endothelial cells, modulating matrix metalloproteinases (MMPs) and TIMPs, and functions as a growth factor in certain tissues via JAK2-STAT3 and MAPK-ERK pathways. Related references: PMID:11460888 PMID:17344214
• In innate immunity, Leptin enhances neutrophil chemotaxis and oxidative burst, increases macrophage phagocytosis and pro-inflammatory cytokine secretion (e.g., IL6, IL8, PGE2), and boosts NK cell cytotoxicity. Related references: PMID:12504075 PMID:15899045 PMID:19688109
• Leptin modulates adaptive immunity by promoting Th1 responses, increasing CD4+CD25- T-cell proliferation, and reducing autophagy during TCR stimulation via mTOR and BCL2 upregulation. Related references: PMID:25060689
• Mutations in LEP are associated with obesity, diabetes mellitus, and other metabolic diseases, and the protein contains key structural features including disulfide bonds and a signal peptide for secretion.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 22-167 of human Leptin (mature protein), suggesting the antibody may recognize the secreted form of Leptin; consider this when designing experiments.
• For IHC-P, perform appropriate antigen retrieval and validate staining in known Leptin-expressing tissues such as adipose tissue or gastric mucosa.
• In ELISA, use recombinant human Leptin standards and ensure the antibody pair is optimized for the specific assay format; cross-reactivity with other species should be verified if applied to non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and labs with a comprehensive range of IVD raw materials, including this anti-Leptin polyclonal antibody for metabolic and obesity research. In addition to validated antibody pairs and bulk reagents, we offer tailored sourcing solutions for rare targets and assay development support to accelerate your project from concept to clinic.