Anti-LEPRE1 (GROS1, OI8) Polyclonal Antibody for WB, ELISA - Q32P28

Core Product Specifications and Parameters

Biological Background: Prolyl 3-hydroxylase 1 Function and Localization

• Also known as Growth suppressor 1 (GROS1), Leprecan-1, and OI8; encoded by the P3H1 (LEPRE1) gene.
• Catalyzes the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences of procollagen chains, a critical step for mature collagen trimer assembly. Related references: PMID:39245686
• May play a role in the cellular secretory pathway and has been reported to exhibit growth suppressive activity in fibroblasts.
• Localizes primarily to the endoplasmic reticulum and is also secreted into the extracellular matrix and space.
• Contains TPR repeat domains and a coiled coil; requires iron ion and vitamin C as cofactors for its dioxygenase activity.
• Post-translational modifications include N-linked glycosylation, and the protein forms disulfide bonds; predicted molecular weight ~83 kDa.
• Associated with skeletal disorders such as osteogenesis imperfecta and dwarfism when mutated.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (amino acids 497–736); antibodies may recognize multiple splice isoforms; validate reactivity in your specific experimental system.
• For Western blot, the target protein runs at approximately 83 kDa; include appropriate reducing agents and confirm the band pattern with relevant controls.
• When using ELISA, consider optimizing the coating antigen concentration and antibody dilution to achieve the desired sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with comprehensive raw-material solutions spanning concept to clinic. This anti-P3H1 polyclonal antibody is suited for investigating collagen biosynthesis, extracellular matrix biology, and skeletal disease mechanisms. Beyond individual reagents, CamelBio supplies validated antibody pairs, bulk ancillary materials, and custom sourcing for rare targets to support your assay development needs.