Anti-LEO1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q8WVC0

Core Product Specifications and Parameters

Biological Background: LEO1 Function and Localization

• LEO1 (RNA polymerase-associated protein LEO1, also known as replicative senescence down-regulated leo1-like protein) is an essential component of the PAF1 complex (PAF1C), a multifunctional transcriptional regulatory complex.
• PAF1C associates with RNA polymerase II via its CTD and is involved in transcriptional elongation, acting independently and synergistically with elongation factors such as TCEA1, DSIF, and HTATSF1.
• LEO1 is required for the transcription of Hox and Wnt target genes, connecting PAF1C to the Wnt signaling pathway.
• PAF1C plays a role in hematopoiesis and leukemogenesis by stimulating KMT2A/MLL1 transcriptional activity, particularly in the context of KMT2A/MLL1-rearranged oncoproteins (e.g., MLL-AF9, MLL-ENL).
• PAF1C regulates histone modifications, including monoubiquitination of histone H2B at Lys-120 (H2BK120ub1) by recruiting the RNF20/40 ubiquitin ligase complex, and subsequent methylation of histone H3 at Lys-4 (H3K4me3).
• LEO1 localizes predominantly to the nucleus.
• LEO1 is highly expressed in skeletal muscle and heart, with weaker expression in placenta and liver.
• During influenza A (H3N2) infection, PAF1C associates with viral NS1 protein, affecting host gene transcription.

Experimental Guidance and Technical Tips

• The immunogen spanning the C-terminal region (amino acids 340-570) may detect full-length LEO1 and relevant isoforms; consider verifying isoform specificity in your experimental system.
• For western blotting, a band at approximately 75 kDa is expected; validate in your sample type and include positive controls with known LEO1 expression (e.g., skeletal muscle, heart).
• For IHC-P, consider antigen retrieval optimization and include tissue controls with high LEO1 expression (skeletal muscle, heart).
• For IF/ICC, confirm nuclear localization and use appropriate fixation/permeabilization conditions.
• ELISA can be used for antigen detection; validate with recombinant protein or cell/tissue lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials and technical services. This LEO1 polyclonal antibody offers a reliable tool for studying gene regulation, chromatin modification, and Wnt signaling pathways. For large-volume antibody procurement, custom polyclonal and monoclonal development, or ancillary reagent sourcing, contact CamelBio to streamline your assay development from concept to clinic.