Anti-Legumain (LGMN) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q99538

Core Product Specifications and Parameters

Biological Background: Legumain Function and Localization

• Legumain (LGMN), also known as asparaginyl endopeptidase (AEP), is a lysosomal cysteine protease that exhibits strict specificity for hydrolysis of asparaginyl bonds and can slowly cleave aspartyl bonds under acidic conditions. Related references: PMID:23776206
• It plays a critical role in the processing of proteins for MHC class II antigen presentation within the lysosomal/endosomal system. Related references: PMID:9872320
• Legumain is also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), promoting antigen translocation in the cytosol (By similarity).
• Required for normal lysosomal protein degradation in renal proximal tubules and for degradation of internalized EGFR, thereby regulating cell proliferation (By similarity).
• The protein is ubiquitous but particularly abundant in kidney, heart, and placenta.
• Subcellularly localized to the lysosome.
• Contains disulfide bonds, is glycosylated, and is synthesized as a zymogen that requires proteolytic activation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 26–288 of human legumain, covering the mature protease domain; this design supports detection of both pro and active forms.
• For Western blotting, the predicted molecular weight is 49 kDa; consider that the proform may migrate at a higher molecular weight. Validate using positive control lysates from kidney or heart.
• In immunohistochemistry (IHC-P), antigen retrieval steps may be required. Tissues such as kidney or placenta, which express high levels of legumain, can serve as positive controls.
• For immunofluorescence (IF/ICC), expect a punctate, lysosomal staining pattern. Co-localization with lysosomal markers is recommended to confirm target specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials for immunology and protease research. For legumain and other protease targets, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents, along with flexible sourcing for rare or custom targets to accelerate your assay development.