Anti-Leghemoglobin (MtLBs) Rabbit Polyclonal Antibody for WB and ELISA - P27992/P27993

Core Product Specifications and Parameters

Biological Background: Leghemoglobin Function and Localization

• Leghemoglobin 6 (also designated leghemoglobin 2, MtLb2, or MtLBs) is a member of the plant hemoglobin family expressed in root nodules of Medicago truncatula.
• Functions as an oxygen carrier, reversibly binding O2 through a pentacoordinated heme iron, facilitating oxygen diffusion to bacteroids while protecting nitrogenase from inactivation (by similarity).
• Essential for symbiotic nitrogen fixation (SNF); also buffers nitric oxide and carbon monoxide, and promotes reactive oxygen species (e.g., H2O2) production (by similarity).
• Subcellularly localizes to the cytosol and nucleus.
• Tissue-specific expression is restricted to root nodules.
• Keywords associated with the protein include: Cytoplasm, Heme, Iron, Metal-binding, Nitration, Nitrogen fixation, Nodulation, Nucleus, Oxygen transport, Phosphoprotein, Reference proteome, Transport.

Experimental Guidance and Technical Tips

• For Western blotting, consider a 15% SDS-PAGE gel to resolve the ~15 kDa leghemoglobin; include pre-stained molecular weight markers for accurate size determination.
• Optimize antibody dilution for each application: typical starting dilutions for WB are 1:500–1:2000; for ELISA, titrate between 0.1–2 µg/mL.
• Validate specificity using recombinant MtLb2 and MtLb6 proteins, as cross-reactivity among leghemoglobin isoforms (P27992/P27993) is possible.
• The immunogen consists of two synthetic peptides; the antibody may detect both native and denatured protein, but denaturing conditions may improve epitope accessibility.
• For ELISA, use peptide-BSA conjugates or purified protein as coating antigen; block non-specific binding with 5% BSA or non-fat milk.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and agricultural research labs with one-stop access to validated IVD raw materials, such as this anti-leghemoglobin polyclonal antibody for plant biomarker detection. Supported by a portfolio that includes optimized antibody pairs, bulk ancillary reagents, and rare target sourcing, CamelBio bridges the gap from concept to application in nitrogen fixation and nodulation studies.