Anti-LEFTY1/2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O75610 / O00292

Core Product Specifications and Parameters

Biological Background: LEFTY1/2 Function and Localization

• Left-right determination factor 1 (LEFTY1, also known as protein lefty-B) is a secreted cytokine belonging to the TGF-beta family.
• It plays an essential role in embryonic left-right axis formation by regulating LEFTY2 and NODAL.
• LEFTY1 is secreted, consistent with its function in intercellular signaling.
• The protein contains a signal peptide and multiple disulfide bonds, characteristic of TGF-beta family members.
• It is glycosylated, which may affect its stability and activity.
• LEFTY1 has been identified through proteomics approaches and is part of the human reference proteome.

Experimental Guidance and Technical Tips

• The immunogen targets a conserved region (aa 267-366) shared by LEFTY1 and LEFTY2, enabling dual recognition; validate in your specific experimental system.
• For Western blot, the predicted protein weight is approximately 34-41 kDa; use appropriate gel concentrations and consider reducing conditions.
• In IHC-P, antigen retrieval conditions (e.g., heat or enzymatic) should be optimized for each tissue type.
• For ELISA, the antibody can be utilized in both direct and sandwich formats; recommended to assess sensitivity with recombinant proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw materials, providing this LEFTY1/2 monoclonal antibody along with a full suite of validated antibody pairs and optimized monoclonal/polyclonal antibodies for developmental biology and cytokine research. We also supply bulk ancillary reagents and specialize in rare target raw-material sourcing, supporting your workflow from concept to clinical application.