Anti-LEFTY1 Rabbit Polyclonal Antibody for WB and ELISA - O75610

Core Product Specifications and Parameters

Biological Background: LEFTY1 Function and Localization

• Left-right determination factor 1 (LEFTY1), also known as left-right determination factor B (LEFTB), protein lefty-1, or lefty-B, is encoded by the LEFTY1 gene.
• LEFTY1 is required for left-right axis determination during embryonic development, acting as a regulator of LEFTY2 and NODAL signaling.
• The protein is secreted and functions as an extracellular signaling molecule in the TGF-beta superfamily.
• Structural features include a signal peptide, disulfide bonds, and glycosylation, classifying it as a cytokine and growth factor.
• The calculated molecular weight is approximately 40.9 kDa (UniProt), while the supplier observes a band at ~41 kDa under denaturing conditions, likely due to glycosylation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 77-366 of human LEFTY1, excluding the signal peptide; this may improve recognition of the mature, processed form.
• For WB, reducing conditions are recommended; the expected band is ~41 kDa. Validate with positive controls such as LEFTY1-overexpressing cells or purified recombinant protein.
• The antibody cross-reacts with mouse LEFTY1, making it suitable for studies in mouse developmental models.
• In ELISA applications, consider pairing with a detection antibody to an alternative epitope for sandwich assays; optimization of coating and detection conditions is advised.
• As LEFTY1 is secreted, sample preparation may require concentrating cell culture supernatant or analyzing serum/plasma with appropriate precautions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material partner, offering everything from validated antibody pairs and optimized polyclonal/monoclonal antibodies to bulk ancillary reagents and rare target sourcing. This anti-LEFTY1 polyclonal antibody addresses the needs of developmental biology research and assay development. Let CamelBio streamline your procurement with reliable supply and technical consulting, supporting your journey from concept to clinic.