Anti-LEF1 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q9UJU2

Core Product Specifications and Parameters

Biological Background: LEF1 Function and Localization

• LEF1 is a nuclear transcription factor that binds DNA in a sequence-specific manner (PubMed:2010090). It plays a central role in the Wnt signaling pathway. Related references: PMID:2010090
• Upon association with CTNNB1 and EP300, LEF1 activates target gene transcription, while TLE family members (TLE1–4) act as repressors (PubMed:11266540). Related references: PMID:11266540
• LEF1 regulates T-cell receptor alpha enhancer function and is required for IL17A-expressing gamma-delta T-cell maturation (PubMed:19653274). Related references: PMID:19653274
• In pancreatic cancer, LEF1 activates MYC and CCND1, promoting proliferation; an isoform lacking the CTNNB1 binding domain antagonizes Wnt signaling.
• CTNNB1-independent functions include activation of fibronectin and repression of E-cadherin promoters, enhancing tumor cell migration and reducing aggregation.
• Localized predominantly to the nucleus, LEF1 is expressed in thymus and overexpressed in colon and pancreatic tumor biopsies.
• Post-translational modifications include phosphorylation and ubiquitination; it is a DNA-binding phosphoprotein implicated in ectodermal dysplasia.
• LEF1 also positively regulates odontoblast differentiation and may contribute to hair cell differentiation and follicle morphogenesis.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted protein weight is ~44 kDa; validate band specificity using lysates from human and mouse cells. Consider using nuclear extracts to enrich LEF1 signal.
• In immunoprecipitation (IP), the recombinant immunogen (aa 1-300) targets the N-terminal region, so it may detect multiple LEF1 isoforms. Confirm capture efficiency with known interacting partners.
• For ELISA, optimize coating conditions and use recombinant protein as a standard to generate quantitative calibration curves.
• Species cross-reactivity has been demonstrated for human and mouse; for other species, sequence homology should be verified before use.
• Given the role in Wnt signaling, this antibody can be employed to probe pathway activation in cancer models; however, empirical validation in each experimental context is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents, supporting diagnostic manufacturers in Wnt signaling and oncology research. Our one-stop sourcing model ensures rapid access to rare target antibodies like anti-LEF1, backed by expert technical and logistical support.