Anti-LEF1 Rabbit Monoclonal Antibody for WB and ELISA - Q9UJU2

Core Product Specifications and Parameters

Biological Background: LEF1 Function and Localization

• LEF1, also known as LEF-1, TCF1-alpha, TCF10, TCF7L3, and TCF1ALPHA, is a member of the TCF/LEF family of transcription factors. Isoforms lacking the CTNNB1 interaction domain may act as antagonists of Wnt signaling.
• LEF1 binds DNA in a sequence-specific manner and participates in the Wnt signaling pathway, activating transcription of target genes in the presence of CTNNB1 and EP300. Related references: PMID:2010090
• TLE1, TLE2, TLE3, and TLE4 repress LEF1-mediated transactivation. Related references: PMID:11266540
• LEF1 regulates T-cell receptor alpha enhancer function. Related references: PMID:19653274
• LEF1 localizes to the nucleus and is detected in thymus. It is highly expressed in colon cancer biopsies and cell lines, and expressed in several pancreatic tumors, with weak expression in normal pancreatic tissue.
• LEF1 is required for IL17A-expressing gamma-delta T-cell maturation, acts as a positive regulator in odontoblast differentiation and hair cell differentiation, and in pancreatic cancer it activates MYC and CCND1 expression to enhance proliferation, while also regulating fibronectin and E-cadherin to promote cell migration.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50-150 of human LEF1, a region conserved in mouse, supporting cross-reactivity.
• For Western blotting, the predicted molecular weight is 44 kDa; empirical migration may vary due to phosphorylation.
• Validate antibody performance in relevant sample systems such as thymus, Jurkat T cells, or colon/pancreatic cancer cell lines.
• Consider isoform-specific expression when interpreting results, as LEF1 has multiple spliced variants.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, supporting diagnostic manufacturers from concept to clinic. This anti-LEF1 monoclonal antibody is part of our comprehensive portfolio for oncology and immunology research, complemented by validated antibody pairs and ancillary reagents. For rare or custom targets, our sourcing expertise ensures reliable supply for your assay development needs.