Anti-LEF1 Polyclonal Antibody for WB, IP, IF-P, ELISA - Q9UJU2

Core Product Specifications and Parameters

Biological Background: LEF1 Function and Localization

• LEF1 (Lymphoid enhancer-binding factor 1) is a sequence-specific DNA-binding transcription factor that participates in the Wnt signaling pathway. Related references: PMID:2010090
• It activates target gene transcription in the presence of CTNNB1 and EP300; TLE family proteins (TLE1-4) repress LEF1/CTNNB1-mediated transactivation. Related references: PMID:11266540
• LEF1 regulates T-cell receptor alpha enhancer function and is required for IL17A-expressing γδ T-cell maturation via BLK modulation (by similarity). Related references: PMID:19653274
• An isoform lacking the CTNNB1 interaction domain may act as a Wnt signaling antagonist.
• In pancreatic cancer, LEF1 transcriptionally activates MYC and CCND1 to enhance proliferation, while also promoting migration by repressing E-cadherin and activating fibronectin in a CTNNB1-independent manner.
• Subcellular localization is predominantly nuclear.
• Tissue specificity: detected in thymus; highly expressed in colon and pancreatic cancer biopsies and cell lines; weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in pancreatic cell lines.
• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitin conjugation. Classified as a DNA-binding transcription factor with roles in Wnt signaling and ectodermal dysplasia.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 20–132 of human LEF1 (NP_057353.1), a region encompassing the N-terminal β-catenin binding domain; the polyclonal nature may allow recognition of full-length LEF1 and isoforms containing this region.
• For Western blot, a band near 44 kDa is expected; validate the band size in your sample system, as post-translational modifications may shift migration.
• The antibody cross-reacts with human, mouse, and rat; verify cross-reactivity in your specific matrix, as sequence homology in the immunogen region influences detection.
• Applications include WB, IP, IF-P (paraffin-embedded immunofluorescence), and ELISA; optimize dilutions and conditions empirically for each assay.
• Since LEF1 is a nuclear transcription factor, nuclear extraction or paraformaldehyde fixation may improve detection in IP and IF-P experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For your LEF1-related transcription and oncology research, we source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials to accelerate your development from concept to clinic. Our B2B sourcing solutions ensure consistent quality and supply for your IVD needs.