Anti-LDL Receptor (LDLR) Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P01130

Core Product Specifications and Parameters

Biological Background: LDL Receptor (LDLR) Function and Localization

• The low-density lipoprotein receptor (LDLR) is a cell surface glycoprotein that mediates the endocytosis of cholesterol-rich LDL particles, playing a central role in maintaining cellular and systemic cholesterol homeostasis.
• LDLR forms a ternary complex with PGRMC1 and TMEM97, which enhances LDL internalization. Related references: PMID:30443021
• The receptor also serves as a cellular entry point for multiple viruses, including hepatitis C virus, vesicular stomatitis virus, HIV-1 (via Tat protein), Crimean-Congo hemorrhagic fever virus, and several alphaviruses.
• LDLR cycles between the plasma membrane, clathrin-coated pits, early and late endosomes, lysosomes, and the Golgi apparatus.
• It undergoes alternative splicing, producing multiple isoforms; mutations in the LDLR gene are linked to familial hypercholesterolemia, a disorder characterized by elevated plasma LDL cholesterol.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation in the EGF-like repeats, phosphorylation, and ubiquitination.
• The protein has a predicted molecular weight of approximately 95 kDa (UniProt: 95,376 Da), consistent with the observed band in Western blotting.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a peptide corresponding to the C-terminal cytoplasmic region (aa 761-860) of human LDLR; it may recognize both full-length and truncated isoforms.
• In Western blotting, a major band at ~95 kDa is expected; additional bands may appear due to glycosylation, phosphorylation, or alternative splicing products.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimal detection may require antigen retrieval; expect membrane and punctate intracellular staining consistent with receptor cycling.
• Since the antibody cross-reacts with mouse and rat LDLR, it can be employed in rodent models; nonetheless, titrate and validate in the relevant species tissue or cell line.
• For ELISA applications, the antibody can be used as a detection reagent; pairing with a suitable capture antibody may improve sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated LDLR antibodies and related reagents for cholesterol metabolism and viral entry studies. Our one-stop sourcing model provides monoclonal/polyclonal antibodies, antigen proteins, and rare-target raw materials, supported by technical consultation to accelerate your assay development pipeline.