Anti-LDHC Polyclonal Antibody for WB, IF-P, ELISA - P07864

Core Product Specifications and Parameters

Biological Background: LDHC Function and Localization

• LDHC (L-lactate dehydrogenase C chain), also known as cancer/testis antigen 32 (CT32), LDH testis subunit, or LDH-X, is a member of the lactate dehydrogenase family that catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+.
• It is specifically expressed in testis and classified as a cancer/testis antigen, potentially making it a target for immunotherapy.
• The protein is reported to play a possible role in sperm motility, contributing to energy metabolism in spermatozoa.
• Subcellular localization is primarily cytoplasmic, where it participates in the glycolytic pathway.
• LDHC is an oxidoreductase that requires NAD as a cofactor, as indicated by UniProt keywords.
• It is a phosphoprotein, subject to phosphorylation, which may regulate its activity or function.
• The protein has a molecular weight of approximately 36 kDa (calculated 36,311 Da) and has been identified in proteomics studies.
• Structural studies (3D-structure keyword) provide insights into its catalytic mechanism and substrate specificity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the internal region (amino acids 150-250) of human LDHC; this region may contribute to the antibody's cross-reactivity with mouse and rat orthologs.
• For Western blot (WB), the expected target weight is ~36 kDa; consider using a gel percentage that resolves proteins in this range and optimize blocking and antibody dilution conditions for your sample type.
• For immunofluorescence (IF-P), permeabilization may be necessary to detect the cytoplasmic localization of LDHC; validate staining specificity using appropriate controls.
• ELISA performance may depend on coating conditions and the detection system; validate with recombinant protein or known positive samples in your laboratory.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This LDHC Polyclonal Antibody supports research into testis-specific protein function and cancer/testis antigen applications. Our sourcing capabilities extend to rare targets and customized solutions, helping you accelerate your project from concept to clinic.