Anti-LDHB Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P07195

Core Product Specifications and Parameters

Biological Background: LDHB Function and Localization

• LDHB encodes the L-lactate dehydrogenase B chain, also known as LDH heart subunit (LDH-H) and renal carcinoma antigen NY-REN-46.
• It catalyzes the reversible interconversion of pyruvate and lactate, coupled with the interconversion of NADH and NAD(+).
• LDHB is predominantly expressed in aerobic tissues, particularly cardiac muscle.
• The LDHB protein localizes to the cytoplasm and the mitochondrial inner membrane.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics.
• Keywords associated with LDHB include Acetylation, Cytoplasm, Membrane, Mitochondrion, NAD, Oxidoreductase, and Phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1-334 of human LDHB, which encompasses nearly the full-length protein. This design likely yields antibodies recognizing multiple linear and conformational epitopes, making it suitable for detecting total LDHB.
• In Western blot (WB), expect a predominant band at approximately 37 kDa, consistent with the predicted molecular weight. Validate the specific band pattern in your experimental system, as post-translational modifications or isoforms may cause slight shifts.
• For immunofluorescence (IF-P) and immunohistochemistry (IHC-P), optimize fixation and permeabilization conditions. LDHB localizes to both cytoplasm and mitochondria; thus, staining patterns may vary by tissue type and metabolic state.
• When using this antibody in ELISA, consider it as a capture or detection reagent after performing a titration to determine optimal working concentrations. Pair with a suitable matched antibody for sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a seamless procurement channel for IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-LDHB product, and bulk ancillary reagents. For projects focused on energy metabolism, cardiac markers, or cancer biomarker discovery, CamelBio can source rare-target antibodies and support your transition from concept to clinic with reliable supply and technical consulting.