Anti-LDHAL6B Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q9BYZ2

Core Product Specifications and Parameters

Biological Background: LDHAL6B Function and Localization

• LDHAL6B (also designated LDHL, LDH6B, or LDHAL6) is a testis-specific member of the lactate dehydrogenase family that catalyzes the interconversion of L-lactate and pyruvate with NAD+ as a coenzyme.
• This enzymatic activity is central to anaerobic glycolysis and cellular energy metabolism, particularly in tissues with high metabolic demands.
• UniProt annotates LDHAL6B with the keywords NAD and Oxidoreductase, classifying it within the reference proteome.
• Tissue specificity analysis indicates predominant expression in testis, implying a specialized role in male germ cell energy homeostasis.
• The predicted molecular mass is approximately 42 kDa (41943 Da), consistent with Western blot detection at this weight.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (aa 1-80) of human LDHAL6B, which is conserved across species; this aligns with observed cross-reactivity with mouse and rat.
• For Western blot, load testis-positive tissue lysates and expect a specific band near 42 kDa; use a suitable blocking and incubation protocol.
• In immunofluorescence (IF-P), testis sections or LDHAL6B-expressing cell models are recommended as positive controls; note that signal may be cytoplasmic.
• Due to homology with other LDH isoforms, verify antibody specificity by including negative controls (e.g., tissues lacking LDHAL6B expression) and/or knockdown experiments.
• Optimal dilutions and conditions should be determined empirically in the end user’s sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic developers from early R&D through clinical application. This anti-LDHAL6B polyclonal antibody exemplifies our catalog of validated antibodies for metabolism and fertility-related biomarkers. Beyond antibodies, we offer bulk ancillary reagents, optimized antibody pairs, and customized rare-target sourcing to streamline your assay development.