Anti-LDHA Rabbit Polyclonal Antibody for IHC-P/IF/ICC/ELISA - P00338

Core Product Specifications and Parameters

Biological Background: LDHA Function and Localization

• LDHA encodes the L-lactate dehydrogenase A chain (also known as LDH-M, cell proliferation-inducing gene 19 protein, or renal carcinoma antigen NY-REN-59), which catalyzes the reversible interconversion of pyruvate and lactate coupled with NADH and NAD+ conversion.
• As a key glycolytic enzyme, LDHA facilitates anaerobic energy production by regenerating NAD+ from NADH during lactate fermentation, supporting ATP generation in oxygen-depleted conditions.
• LDHA is predominantly expressed in skeletal muscle and liver, tissues with high anaerobic metabolic activity.
• The protein localizes to the cytoplasm, consistent with its metabolic role in glycolysis.
• Post-translational modifications include acetylation and phosphorylation, and the protein is subject to ubiquitin-like conjugation, which may regulate its activity or stability.
• Genetic variations in LDHA are associated with glycogen storage disease type XI, a metabolic disorder characterized by exercise intolerance and myoglobinuria.
• Alternative splicing of the LDHA gene produces multiple isoforms, potentially contributing to tissue-specific regulation of lactate metabolism.
• LDHA encodes the muscle-type subunit (LDH-M) of the tetrameric lactate dehydrogenase enzyme, which can combine with LDHB to form five distinct isoenzymes with varying kinetic properties.

Experimental Guidance and Technical Tips

• The immunogen sequence corresponds to the N-terminal region (aa 1-100) of human LDHA; consider validating the antibody’s specificity in your experimental context using positive and negative controls.
• For IHC-P, optimize antigen retrieval and primary antibody dilution; tissues with high LDHA expression (e.g., skeletal muscle, liver) should show robust cytoplasmic staining.
• In IF/ICC, ensure proper cell fixation and permeabilization to detect the cytoplasmic LDHA signal; co-staining with organelle markers may assist in confirming localization.
• For ELISA, test the antibody in both direct and sandwich formats with appropriate standards and validate performance across the expected concentration range.
• Given the antibody's reported cross-reactivity with mouse LDHA, it may be suitable for studies in mouse models; however, confirm cross-reactivity in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, from validated antibodies and antigens to ancillary reagents. Our one-stop platform supports the development of glycolytic enzyme assays and cancer metabolism research by offering high-quality anti-LDHA antibodies and related reagents. For custom or bulk requirements, our team can source optimized antibody pairs and rare-target materials to advance your diagnostic projects from concept to clinic.