Anti-LDHA Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P00338

Core Product Specifications and Parameters

Biological Background: LDHA Function and Localization

• LDHA, also known as LDH muscle subunit (LDH-M), cell proliferation-inducing gene 19 protein (PIG19), and renal carcinoma antigen NY-REN-59, is the L-lactate dehydrogenase A chain encoded by the LDHA gene.
• It catalyzes the stereospecific interconversion of pyruvate and lactate, coupled with the interconversion of NADH and NAD(+), playing a central role in anaerobic glycolysis.
• LDHA is predominantly localized in the cytoplasm.
• The protein is highly expressed in anaerobic tissues, notably skeletal muscle and liver.
• Post-translational modifications include acetylation, phosphorylation, and isopeptide bond formation, as well as alternative splicing.
• Mutations in LDHA are associated with glycogen storage disease.
• The predicted molecular weight is ~36.7 kDa, but observed bands range 26–39 kDa due to modifications and isoforms.
• Keywords: oxidoreductase, NAD, phosphoprotein, acetylation, alternative splicing, disease variant.

Experimental Guidance and Technical Tips

• The antibody was raised against full-length recombinant human LDHA (residues 1–332), suggesting recognition of both native and denatured forms.
• Knockdown validation confirms specificity; we recommend including appropriate positive and negative controls (e.g., siRNA-treated lysates) in your experiments.
• Suitable applications include Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA. For IF/ICC, optimize permeabilization and fixation conditions for your cell type.
• The observed band in WB for endogenous LDHA is typically between 26 kDa and 39 kDa; validate the expected molecular weight in your sample system.
• Cross-reactivity has been demonstrated in human, mouse, and rat samples; for other species, sequence alignment and testing are advised.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material partner, CamelBio offers this anti-LDHA monoclonal antibody alongside a wide range of validated antibodies, antigens, and ancillary reagents for metabolic and oncology research. From concept to clinic, we support diagnostic manufacturers and laboratories with reliable, high-quality raw materials and technical services to accelerate your assay development.