Anti-LDHA Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P00338

Core Product Specifications and Parameters

Biological Background: LDHA Function and Localization

• LDHA (Lactate Dehydrogenase A), also known as LDHM, GSD11, PIG19, or HEL-S-133P, is a key glycolytic enzyme.
• It catalyzes the stereospecific interconversion of pyruvate and lactate, coupled with the oxidation/reduction of NADH/NAD+.
• LDHA is the muscle subunit (LDH-M) of the tetrameric LDH enzyme, predominantly forming the LDH-5 isoenzyme in anaerobic tissues.
• The protein localizes to the cytoplasm and is highly expressed in skeletal muscle and liver, reflecting its role in anaerobic glycolysis.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, which may regulate its activity and stability.
• Mutations in LDHA are associated with glycogen storage disease XI (GSD11), underscoring its metabolic importance.
• The antibody target spans amino acids 1-332, covering the full-length protein, and is expected to detect all major isoforms.
• Keywords: Acetylation, Alternative splicing, Cytoplasm, Direct protein sequencing, Disease variant, Glycogen storage disease, Isopeptide bond, NAD, Oxidoreductase, Phosphoprotein, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant protein covering amino acids 1-332 of human LDHA (NP_005557.1), making this antibody suitable for detecting total LDHA protein levels.
• For Western blotting (WB), expect a band between 26-39 kDa, consistent with the calculated molecular weight of ~37 kDa; validate in your specific sample system.
• This polyclonal antibody is also applicable in IHC-P, IF/ICC, IP, and ELISA; optimal dilutions should be determined empirically for each assay.
• The antibody cross-reacts with human, mouse, and rat LDHA, based on sequence homology; consider verifying reactivity in other species if needed.
• LDHA is highly expressed in skeletal muscle and liver; use appropriate positive and negative control tissues for immunohistochemistry.
• For functional studies, note that LDHA activity is modulated by post-translational modifications; this antibody may not distinguish modified forms without additional enrichment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with comprehensive IVD raw material access—from validated LDHA antibodies and recombinant proteins to ancillary reagents. As your sourcing partner for glycolysis and cancer metabolism targets, we offer polyclonal and monoclonal options, bulk customizable lots, and expedited rare-material sourcing to streamline your assay development from concept to clinic.