Anti-LDHA Monoclonal Antibody for WB and ELISA - P00338

Core Product Specifications and Parameters

Biological Background: LDHA Function and Localization

• LDHA (L-lactate dehydrogenase A chain) is also known as cell proliferation-inducing gene 19 protein, LDH muscle subunit (LDH-M), and renal carcinoma antigen NY-REN-59.
• It catalyzes the stereospecific interconversion of pyruvate and lactate with the simultaneous interconversion of NADH and NAD(+), a key step in anaerobic glycolysis.
• Alternative splicing generates multiple transcript variants, contributing to functional diversity.
• The protein is predominantly located in the cytoplasm, consistent with its role in glycolysis.
• Tissue-specific expression is observed predominantly in anaerobic tissues such as skeletal muscle and liver.
• Post-translational modifications include acetylation, phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, which may regulate enzymatic activity or protein stability.
• Keywords associated with the protein include 3D-structure, disease variant (glycogen storage disease), and direct protein sequencing, highlighting its structural and clinical significance.
• The molecular weight of the monomeric form is approximately 36.7 kDa, consistent with the observed 26-39 kDa range in denaturing gels.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-100 of human LDHA (NP_005557.1), located at the N-terminal region; this design typically favors detection of the denatured protein in Western blotting.
• For WB, consider using whole-cell lysates from human, mouse, or rat tissues rich in LDHA (e.g., skeletal muscle or liver) and validate band specificity with appropriate controls.
• In ELISA, the antibody may be suitable for capturing or detecting LDHA; optimization with recombinant protein or a verified native sample is recommended.
• The reported molecular weight of 26-39 kDa may reflect monomeric LDHA, but note that native LDH enzymes exist as tetramers (~140 kDa) under non-denaturing conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw-material sourcing, offering a comprehensive range of monoclonal antibodies, validated antibody pairs, and ancillary reagents for diagnostic development. This anti-LDHA rabbit monoclonal antibody supports research into glycolysis and metabolic disorders, and we can further assist with bulk orders, custom formulations, and rare target sourcing. From concept to clinic, CamelBio provides integrated technical services and supply-chain solutions to accelerate your IVD projects.