Anti-LDB3 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - O75112

Core Product Specifications and Parameters

Biological Background: LDB3 Function and Localization

• LDB3 (also known as ZASP or Cypher) is a LIM domain-binding protein that functions as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton.
• It localizes to the sarcomeric Z-line, where it interacts with α-actinin and other Z-disc proteins, playing a critical role in myofibril assembly and maintenance.
• Alternative splicing generates multiple isoforms that are differentially expressed, contributing to the structural and signaling versatility of the protein.
• LDB3 is predominantly expressed in skeletal muscle, with lower expression in the heart, and detectable levels in brain and placenta.
• The protein contains LIM domains, a PDZ domain, and a ZASP-like motif, enabling protein-protein interactions and zinc binding.
• Mutations in LDB3 are associated with various cardiomyopathies and myopathies, including dilated cardiomyopathy (CMD1C), hypertrophic cardiomyopathy (CMH24), and myofibrillar myopathy (MFM4), highlighting its clinical relevance.
• Post-translational modifications include phosphorylation and methylation, which may regulate its function and localization.
• LDB3 is also found in perinuclear regions and pseudopodia, suggesting potential roles in cell signaling and motility beyond the sarcomere.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-283), suggesting that the antibody may recognize multiple LDB3 isoforms sharing this sequence. For isoform-specific detection, validate with known positive controls.
• For Western blotting, the expected band size is approximately 77 kDa (as per the provided Protein Weight), but actual migration may vary due to alternative splicing and post-translational modifications. Use a molecular weight marker and compare with predicted sizes.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), LDB3 localizes to Z-lines in striated muscle, yielding a characteristic striated staining pattern. Counterstaining with phalloidin can help visualize sarcomeric organization.
• Validate cross-reactivity in mouse and rat tissues by comparing with human samples; slight variations in signal intensity may occur.
• For ELISA, consider using a recombinant LDB3 protein as a standard to assess binding sensitivity and assay robustness.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing one-stop access to validated antibodies like this anti-LDB3 polyclonal antibody. Our portfolio includes optimized monoclonal and polyclonal antibodies, matched antigen pairs, bulk ancillary reagents, and rare target sourcing—supporting your muscle disease and cardiomyopathy research from early discovery to clinical application. With a commitment to quality and supply continuity, CamelBio helps diagnostics developers accelerate their pipeline efficiently.