Anti-LDB1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q86U70

Core Product Specifications and Parameters

Biological Background: LDB1 Function and Localization

• LDB1 is a transcriptional co-regulator that binds the LIM domain of various LIM-domain-containing transcription factors, modulating their transcriptional activity through specific partner interactions.
• Plays a critical role in neuronal development by cooperating with LHX3 and ISL1 to specify interneurons and motor neurons.
• Involved in early embryonic axis formation, acting synergistically with LHX1/LIM1 to activate gene expression.
• Essential for erythroid development; interacts with LMO2 to maintain erythroid precursors in an immature state.
• Localizes predominantly to the nucleus, consistent with its function as a transcriptional regulator.
• Ubiquitously expressed across adult tissues, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, lung, and peripheral blood leukocytes.
• Subject to post-translational modifications such as acetylation, phosphorylation, and ubiquitination, and undergoes alternative splicing, generating multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–50 at the N-terminus, a region likely conserved across human, mouse, and rat, supporting observed cross-reactivity. Verify sequence alignment for your specific experimental model.
• For Western blot, a band around 47 kDa is expected; be aware that post-translational modifications or alternative splicing may yield additional bands.
• When using for IHC-P, optimize antigen retrieval conditions and antibody dilution; validation in the relevant tissue system is advised.
• ELISA applications may require pairing with a suitable detection antibody; consider in-house validation for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, supporting projects from initial concept through clinical application. For researchers focusing on transcriptional regulation and developmental biology, we offer a comprehensive portfolio including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our rare-target raw-material sourcing capabilities further empower your diagnostic development, ensuring you have reliable access to the critical reagents you need.