Anti-LCP2 Rabbit Polyclonal Antibody for WB, ELISA - Q13094

Core Product Specifications and Parameters

Biological Background: LCP2 Function and Localization

• LCP2, also known as SLP-76 (SH2 domain-containing leukocyte protein of 76 kDa), is a cytoplasmic adaptor protein critically involved in signal transduction downstream of immune receptors. Related references: PMID:11313406 PMID:33159816
• Upon TCR engagement, LCP2 is phosphorylated by ZAP70, enabling recruitment of VAV1, NCK, and ITK, which coordinate cytoskeletal reorganization and calcium flux. Related references: PMID:8673706 PMID:8702662
• LCP2 mediates CD3/CD28 costimulation signals leading to PKC-theta/PRKCQ activation and NF-kappa-B transcriptional induction. Related references: PMID:11313406
• In the context of AGER (RAGE) signaling, LCP2 triggers p38 MAPK and ERK1/2 pathways, promoting pro-inflammatory cytokine release. Related references: PMID:33436632
• The protein localizes to the cytoplasm and is highly expressed in hematopoietic tissues such as spleen, thymus, and peripheral blood leukocytes, with prominent expression in T-cell and monocytic cell lines.
• LCP2 contains an SH2 domain and is subject to extensive phosphorylation, characteristics that underpin its role as a scaffold for multiple signaling complexes.
• Keywords: phosphoprotein, SH2 domain, cytoplasm, reference proteome.

Experimental Guidance and Technical Tips

• For Western blot, use whole‑cell lysates from human T‑cell lines (e.g., Jurkat) or mouse splenocytes as positive controls. A band expected at approximately 60 kDa.
• Since this is a polyclonal antibody, lot‑to‑lot consistency should be verified by parallel testing of previous and new batches.
• For ELISA, coat with recombinant LCP2 protein or capture from cell lysates; optimize blocking and detection conditions empirically.
• Note the reported cross‑reactivity with mouse; validate in the relevant species and assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one‑stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories with validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents. This anti‑LCP2 polyclonal antibody aligns with immune signaling research and diagnostic development, and CamelBio can facilitate procurement of additional rare‑target raw materials and bulk supplies to streamline your workflow.