Anti-LCK Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P06239

Core Product Specifications and Parameters

Biological Background: LCK Function and Localization

• LCK is a non-receptor tyrosine-protein kinase containing SH2 and SH3 domains, encoded by the LCK gene.
• It plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Related references: PMID:2470098
• LCK is a key mediator of T-cell antigen receptor (TCR)-linked signal transduction, constitutively associated with CD4 and CD8 cytoplasmic tails, and phosphorylates ITAMs on TCR chains to initiate signaling. Related references: PMID:2470098, PMID:40592325
• Following TCR engagement, LCK phosphorylates and activates ZAP70, recruiting downstream signaling molecules that ultimately lead to lymphokine production. Related references: PMID:2470098
• LCK also participates in IL2 receptor-linked signaling and interacts with FYB2, contributing to T-cell proliferative responses. Related references: PMID:27335501
• The protein localizes to the cell membrane and cytosol (UniProt subcellular location: Cell membrane, Cytoplasm, cytosol).
• LCK is expressed specifically in lymphoid cells and undergoes extensive post-translational modifications, including alternative splicing, myristoylation, palmitoylation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (aa 1–210) of human LCK, including SH3 and SH2 domains; this design may recognize both full-length and certain splice variants, so validate specificity in your sample system.
• For Western blotting, the predicted molecular weight is approximately 58 kDa; optimize gel percentage and transfer conditions accordingly.
• LCK is expressed specifically in lymphoid cells; consider using Jurkat T-cell lysates as a positive control for human LCK detection.
• For immunofluorescence (IF-P), optimize antibody concentration and cell permeabilization protocols for membrane-associated targets.
• This antibody reacts with human and mouse LCK; confirm cross-reactivity in mouse lymphoid tissues if required.
• As LCK participates in TCR signaling, T-cell activation may alter its phosphorylation status; carefully control stimulation conditions in functional studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD manufacturers and researchers developing LCK-related immunoassays, including this validated anti-LCK rabbit monoclonal antibody. Our portfolio supports T-cell signaling research with optimized antibody pairs, bulk ancillary reagents, and rare target raw-material procurement to streamline your assay development from concept to clinic.