Anti-LCAT Monoclonal Antibody for WB, ELISA - P04180

Core Product Specifications and Parameters

Biological Background: LCAT Function and Localization

• LCAT is a secreted enzyme that plays a central role in extracellular cholesterol metabolism by catalyzing the transfer of fatty acids from phosphatidylcholines (lecithins) to free cholesterol, generating cholesteryl esters and lysophosphatidylcholines on the surface of high-density lipoproteins (HDLs) and low-density lipoproteins (LDLs) (PubMed:10329423, PubMed:19065001, PubMed:26195816). Related references: PMID:10329423 PMID:19065001 PMID:26195816
• The enzyme exhibits substrate specificity for plasma 16:0-18:2 and 18:0-18:2 phosphatidylcholines (PubMed:8820107). Related references: PMID:8820107
• In the brain, LCAT is produced by astrocytes and esterifies cholesterol on nascent APOE-containing lipoproteins, influencing cerebrospinal fluid APOE and APOA1 levels and contributing to lipoprotein maturation (PubMed:10722751). Related references: PMID:10722751
• LCAT also functions as a platelet-activating factor (PAF) acetylhydrolase, catalyzing the hydrolysis of PAF to lyso-PAF and the transacetylation of PAF to 1-hexadecanoyl-sn-glycero-3-phosphocholine (PubMed:8016111). Related references: PMID:8016111
• Additionally, LCAT catalyzes the esterification of (24S)-hydroxycholesterol (cerebrosterol) to form 24(S)OH-cholesterol monoesters, potentially important in brain sterol metabolism (PubMed:24620755). Related references: PMID:24620755
• LCAT is a secreted glycoprotein with a signal peptide and contains disulfide bonds. It has a molecular weight of approximately 50 kDa (UniProt: 49578 Da; product specification: 50 kDa).
• Expression is highest in liver, brain, and testes, and the protein is detectable in blood plasma and cerebrospinal fluid.
• Mutations in LCAT are associated with familial LCAT deficiency and fish-eye disease, highlighting its critical role in lipoprotein metabolism.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the N-terminal region (amino acids 1–100) of human LCAT. Verify that your target of interest contains this epitope.
• For western blot (WB) analysis, a band around 50 kDa is expected. Consider using a positive control such as human plasma or liver lysate, and optimize protein loading and antibody dilution for your system.
• The antibody is reported to cross-react with mouse and rat LCAT. If using the antibody for cross-species applications, validate specificity and sensitivity in the relevant sample system.
• For ELISA applications, this antibody may be used for quantitative detection of LCAT in biological samples. Include appropriate standards and optimize dilutions to ensure assay linearity and accuracy.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories with comprehensive solutions from concept to clinic. This LCAT antibody supports research into cardiovascular disease, lipid metabolism, and related disorders. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, streamlining your assay development supply chain.