Anti-LC3B Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q9GZQ8

Core Product Specifications and Parameters

Biological Background: LC3B Function and Localization

• LC3B (microtubule-associated protein 1 light chain 3 beta) is a ubiquitin-like modifier essential for autophagosome formation, acting as a core component of the autophagy machinery. Related references: PMID:20418806, PMID:23209295, PMID:28017329
• LC3B plays a critical role in mitophagy, anchoring autophagolysosomes to damaged mitochondria via C-18 ceramide binding, thereby regulating mitochondrial quality and ROS levels. Related references: PMID:22922758, PMID:23209295, PMID:28017329
• It promotes primary ciliogenesis by mediating autophagic removal of OFD1 from centriolar satellites. Related references: PMID:24089205
• LC3B facilitates endoplasmic reticulum remodeling during nutrient stress by interacting with the reticulophagy receptor TEX264, leading to ER-phagy. Related references: PMID:31006537, PMID:31006538
• Upon nutrient stress, LC3B recruits JMY to phagophore membranes, enhancing actin nucleation and autophagosome biogenesis. Related references: PMID:30420355
• LC3B is expressed most abundantly in heart, brain, skeletal muscle, and testis, with low expression in liver.
• Subcellularly, LC3B localizes to autophagosome membranes, mitochondrial membranes, and the cytoskeleton, consistent with its roles in autophagy and trafficking.
• As a member of the LC3/GABARAP family, LC3B is conjugated to phosphatidylethanolamine during autophagy, a process commonly monitored as a marker for autophagic flux.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 2-107 of human LC3B. This N-terminal region may detect both unconjugated (LC3B-I) and lipidated (LC3B-II) forms; validate in relevant sample systems.
• For Western blot, a band near 15 kDa (LC3B-II) and another near 18 kDa (LC3B-I) may be observed; confirm molecular weight shift under your conditions.
• LC3B is a hallmark autophagy marker; use known inducers (starvation, rapamycin) and inhibitors (chloroquine) to verify autophagic flux detection.
• The antibody cross-reacts with human, mouse, and rat; verify other species before use.
• In immunofluorescence, expect punctate LC3B staining upon autophagy induction; co-stain with lysosomal markers for co-localization analysis.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material and technical service provider, CamelBio supports diagnostic manufacturers and research institutes with validated antibody tools like this LC3B monoclonal antibody for autophagy research. We offer a comprehensive supply chain including bulk antibody pairs, ancillary reagents, and rare target material sourcing, streamlining your development from concept to clinic.