Anti-LC3B Monoclonal Antibody for WB, ELISA - Q9GZQ8

Core Product Specifications and Parameters

Biological Background: LC3B Function and Localization

• LC3B, also known as microtubule-associated protein 1 light chain 3 beta (MAP1LC3B) or autophagy-related protein ATG8F, is a core component of the autophagy machinery.
• It functions as a ubiquitin-like modifier in the early stages of phagophore elongation during autophagosome biogenesis; the related GABARAP/GATE-16 proteins are required for later maturation steps.
  Related references:
  PMID:20418806
  PMID:23209295
  PMID:28017329
• In mitophagy, LC3B binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes, thereby promoting the selective degradation of damaged mitochondria and regulating mitochondrial quality.
  Related references:
  PMID:22922758
  PMID:23209295
  PMID:28017329
• It contributes to primary ciliogenesis by mediating the autophagic removal of OFD1 from centriolar satellites.
  Related references:
  PMID:24089205
• LC3B interacts with the reticulophagy receptor TEX264 to remodel subdomains of the endoplasmic reticulum into autophagosomes during nutrient stress, facilitating ER turnover.
  Related references:
  PMID:31006537
  PMID:31006538
• Upon nutrient deprivation, LC3B directly recruits the actin nucleation-promoting factor JMY to the phagophore membrane, stimulating actin polymerization and driving autophagosome biogenesis.
  Related references:
  PMID:30420355
• The protein localizes primarily to autophagosome membranes, cytoplasmic vesicles, the endomembrane system, the mitochondrial outer membrane, and the cytoskeleton.
• Its expression is highest in heart, brain, skeletal muscle, and testis, with minimal levels observed in the liver.

Experimental Guidance and Technical Tips

• Since the immunogen spans amino acids 1–125 of human LC3B, the antibody may recognize both the non-lipidated LC3B-I and the lipidated LC3B-II forms; include appropriate molecular weight markers and validate in your experimental system to distinguish autophagy flux.
• For western blotting, a ~15 kDa band is expected; consider using a reducing SDS-PAGE gel and confirm band specificity in relevant human, mouse, or rat samples.
• When performing ELISA, optimize antibody dilution and coating conditions for the specific assay format; validate with recombinant or cell-derived LC3B antigen.
• Cross-reactivity with endogenous LC3B in mouse and rat is reported; test in positive and negative control lysates to ensure signal linearity in your model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research laboratories, and institutes a streamlined single-source solution for IVD raw materials, technical services, and consulting—covering every phase from concept to clinical application. Our supply portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to support your autophagy and microtubule-associated protein studies. Contact us to explore how our one-stop approach can accelerate your assay development.