Anti-LC3A/LC3B Rabbit PolymAb® for WB, IHC-P, IF/ICC, ELISA - Q9GZQ8 / Q9H492

Core Product Specifications and Parameters

Biological Background: LC3A/LC3B Function and Localization

• Microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), also known as LC3B, is a ubiquitin-like modifier essential for autophagosome formation. Related references: PMID:20418806, PMID:23209295, PMID:28017329

• LC3B plays a critical role in mitophagy by binding C-18 ceramides and anchoring autophagolysosomes to outer mitochondrial membranes, facilitating the elimination of damaged mitochondria. Related references: PMID:22922758

• The autophagy-related LC3 subfamily, which includes LC3A and LC3B, is primarily involved in the elongation of the phagophore membrane, whereas the GABARAP/GATE-16 subfamily is essential for autophagosome maturation. Related references: PMID:20418806, PMID:23209295, PMID:28017329

• LC3B promotes primary ciliogenesis by mediating the autophagic removal of OFD1 from centriolar satellites. Related references: PMID:24089205

• Through interaction with the reticulophagy receptor TEX264, LC3B participates in the selective autophagic degradation of endoplasmic reticulum subdomains under nutrient stress. Related references: PMID:31006537, PMID:31006538

• Upon nutrient stress, LC3B directly recruits the actin nucleation factor JMY to phagophore membranes, stimulating actin polymerization and autophagosome biogenesis. Related references: PMID:30420355

• LC3B localizes to autophagosome membranes, the mitochondrial outer membrane, the cytoskeleton, and cytoplasmic vesicles, reflecting its dynamic roles in autophagy.

• The protein is most abundant in heart, brain, skeletal muscle, and testis, with little expression in liver, and is subject to lipidation and phosphorylation as key regulatory modifications.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic peptide derived from human LC3A/LC3B; thus, reactivity with both LC3A and LC3B is expected. For Western blotting, the predicted protein weight is ~14 kDa; consider confirming band shifts indicative of LC3-I to LC3-II conversion upon autophagy induction.

• In immunofluorescence and immunohistochemistry, autophagosome puncta may be enhanced under starvation or stress conditions; optimize fixation and permeabilization protocols to preserve the autophagosomal membrane signal.

• For ELISA and other quantitative applications, validate the antibody in your specific sample system and treatment conditions to ensure specificity and linear dynamic range.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-LC3A/LC3B antibody supports autophagy research and autophagosome detection across multiple applications. For rare target sourcing and tailored supply solutions, contact CamelBio to bridge your concept-to-clinic needs.