Anti-LC3A/LC3B Rabbit Polyclonal Antibody for WB - Q9GZQ8/Q9H492

Core Product Specifications and Parameters

Biological Background: LC3A/LC3B Function and Localization

• Microtubule-associated protein 1 light chain 3 beta (LC3B, also known as ATG8F) is a ubiquitin-like modifier that plays a central role in autophagy, specifically in the formation of autophagosomal vacuoles (autophagosomes). Related references: PMID:20418806, PMID:23209295, PMID:28017329
• LC3B is critical for mitophagy, a selective autophagy process that removes damaged mitochondria; upon mitochondrial fission and cellular stress, LC3B binds C-18 ceramides to anchor autophagolysosomes to outer mitochondrial membranes. Related references: PMID:23209295, PMID:28017329, PMID:22922758
• LC3B promotes primary ciliogenesis by clearing OFD1 from centriolar satellites via the autophagic pathway. Related references: PMID:24089205
• Through interaction with the reticulophagy receptor TEX264, LC3B participates in endoplasmic reticulum remodeling into autophagosomes during nutrient stress, which subsequently fuse with lysosomes for ER turnover. Related references: PMID:31006537, PMID:31006538
• Upon nutrient stress, LC3B directly recruits the actin nucleation factor JMY to phagophore membrane surfaces, promoting actin nucleation and autophagosome biogenesis. Related references: PMID:30420355
• Subcellularly, LC3B is found on autophagosome membranes, cytoplasmic vesicles, the endomembrane system, mitochondrial membranes, and the cytoskeleton.
• LC3B is most abundantly expressed in heart, brain, skeletal muscle, and testis, with little expression in liver.
• LC3B is classified under the autophagy and ubiquitin-like conjugation pathway; it exists as a lipoprotein and phosphoprotein, highlighting its regulatory modifications. This antibody also recognizes LC3A (MAP1LC3A), a closely related paralog with conserved functions in autophagy.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminus (aa 1-60) of human LC3A, a region highly conserved with LC3B. Expect cross-reactivity with both LC3A and LC3B forms due to sequence homology.
• In Western blotting, both the unlipidated (LC3-I) and lipidated (LC3-II) forms may be detected; LC3-II migrates slightly lower (~14-16 kDa) on SDS-PAGE. Perform appropriate controls (e.g., autophagy induction by starvation or inhibitors) to distinguish the forms.
• For immunofluorescence applications, optimize fixation and permeabilization conditions, as LC3 often decorates autophagic structures (puncta). Co-staining with lysosomal markers may help confirm autophagic flux.
• In immunohistochemistry, antigen retrieval may be required due to the epitope location. Validate antibody specificity using si/shRNA knockdown or blocking peptide where feasible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined procurement solution for IVD raw materials. This LC3A/LC3B polyclonal antibody is suitable for autophagy-related research and assay development. As a single-source partner, CamelBio can also supply validated antibody pairs, bulk ancillary reagents, and assist in sourcing rare-target raw materials to accelerate your projects from concept to clinic.